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May 2011

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Subject:
From:
Carole Genovesi <[log in to unmask]>
Reply To:
The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>
Date:
Fri, 29 Apr 2011 12:46:11 -0700
Content-Type:
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any chance of getting a copy of this?  i don;t have axccess to that journal
C. Genovesi
Sr. Pharm Specialist
Genentech, Inc.
[log in to unmask]






On Wed, Apr 27, 2011 at 10:23 AM, Tim Sandle <[log in to unmask]> wrote:

>
> [virus checked]
> Carole's advice is absolutely correct. Although R2A is theoretically better
> (and practically in my experience), you really need to run a comparative
> study to determine the appropriate media, incubation temperature and time,
> for your particular medium.
> I contributed to a paper which reported the outcome of such a study. It
> might be useful for those who wish to refer to it. The reference is:
> Sandle, T. and Skinner, K. (2005): &#8216;Examination of the optimal
> cultural conditions for the microbiological analysis of a cold demineralised
> water system in a pharmaceutical manufacturing facility&#8217;, European
> Journal of Parenteral and Pharmaceutical Sciences, Vol. 10, No.1, pp9-14
> Tim Sandle, UK
>
> -----The Pharmaceutical Microbiology Forum Email List <
> [log in to unmask]> wrote: -----
> To: [log in to unmask]
> From: Carole Genovesi
> Sent by: The Pharmaceutical Microbiology Forum Email List
> Date: 27/04/2011 17:32
> Subject: Re: [PMFLIST] USP Purified Water Microbial Testing
>
> [virus checked]
> I am really becoming concerned that everyone is assuming that R2A is the
> best media for recovering organisms from process water systems.  You really
> need to perform a study using both media (or all 3 if you include PCA) to
> determine which media best supports the organisms found in your particular
> water system(s).  Having done this at 3 different (widely separated
> geographically)  plants, one found R2A worked better, one is using PCA and
> one is using TSA.  The problem with R2A is the 5 day incubation - do you
> really have a leeway of 5 days before responding to a detected problem? Do
> you have the surplus of personnel to do an interim read?
>
> C. Genovesi
>
>
>
>
>
>
>
> On Mon, Apr 25, 2011 at 1:17 PM, Peter Lisecki <[log in to unmask]
> >wrote:
>
> > Thilak,   Do you mind explaining what you mean by more favorable?  Did
> you
> > try other media when you were validating your water system?  I have not
> > seen
> > any documentation, that shows TSA as better than media with less
> nutrients.
> > It would be interesting, if that were the case.  Peter
> >
> > On Mon, Apr 25, 2011 at 1:04 AM, Tilak Kulasuriya <
> > [log in to unmask]> wrote:
> >
> > > Hi Peter,
> > > Yes you can . We in GSK use TSA. It is more favourable.
> > >
> > > Warm Regards,
> > > Thilak
> > >
> > >
> > >
> > > -----Original Message-----
> > > From: The Pharmaceutical Microbiology Forum Email List [mailto:
> > > [log in to unmask]] On Behalf Of Peter Chan
> > > Sent: 21 April 2011 21:02
> > > To: [log in to unmask]
> > > Subject: [PMFLIST] USP Purified Water Microbial Testing
> > >
> > >  Hello Forum,
> > > Can i use the TSA agar instead of pour plate agar for USP purified
> water
> > > microbial testing?  What is the difference between these two agars?
> > > Thank you in advance for your help
> > > peter
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> > > NovaTek International - http://www.ntint.com
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> > > =================================
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> >
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> >
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> >
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> >
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> >
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> >
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> >
> > Millipore - http://www.millipore.com
> >
> > MODA Technology Partners: now a LONZA company http://lonza.com/moda
> >
> > Pall - http://www.pall.com
> >
> > NovaTek International - http://www.ntint.com
> >
> > Rapid Micro Biosystems - http://www.rapidmicrobio.com  (formerly GPS)
> >
> > Veltek Associates, Inc - http://www.sterile.com
> >
> > =================================
> > The nature of this service is to provide a medium for communication.  The
> > specific statements and endorsements of individuals participating in the
> > discussions are not necessarily those of The Microbiology Network, Inc.,
> the
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> >
>
> ------------------
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>
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>
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>
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>
> Biolog - http://www.biolog.com
>
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>
> Biotest - http://www.BiotestUSA.com/micro
>
> MicroBioLogics, Inc. - http://www.microbiologics.com
>
> Millipore - http://www.millipore.com
>
> MODA Technology Partners: now a LONZA company http://lonza.com/moda
>
> Pall - http://www.pall.com
>
> NovaTek International - http://www.ntint.com
>
> Rapid Micro Biosystems - http://www.rapidmicrobio.com  (formerly GPS)
>
> Veltek Associates, Inc - http://www.sterile.com
>
> =================================
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> ------------------
> The PMFList (http://microbiol.org/PMFList_info.htm) is operated from
> The Microbiology Network (http://microbiol.org) and supported by
> our sponsors (http://microbiol.org/sponsor.htm) as a service to
> the scientific community.
>
> Please take a second to visit our sponsors' web sites and say thank you for
> their support of this service.
>
> Accugenix - http://www.accugenix.com
>
> American Type Culture Collection - http://www.atcc.org
>
> BD Diagnostic Systems - http://www.bd.com/ds/
>
> Biolog - http://www.biolog.com
>
> Biomerieux - http://industry.biomerieux-usa.com
>
> Biotest - http://www.BiotestUSA.com/micro
>
> MicroBioLogics, Inc. - http://www.microbiologics.com
>
> Millipore - http://www.millipore.com
>
> MODA Technology Partners: now a LONZA company http://lonza.com/moda
>
> Pall - http://www.pall.com
>
> NovaTek International - http://www.ntint.com
>
> Rapid Micro Biosystems - http://www.rapidmicrobio.com  (formerly GPS)
>
> Veltek Associates, Inc - http://www.sterile.com
>
> =================================
> The nature of this service is to provide a medium for communication.  The
> specific statements and endorsements of individuals participating in the
> discussions are not necessarily those of The Microbiology Network, Inc., the
> PMF, or the sponsors of the list.
>

------------------
The PMFList (http://microbiol.org/PMFList_info.htm) is operated from
The Microbiology Network (http://microbiol.org) and supported by
our sponsors (http://microbiol.org/sponsor.htm) as a service to
the scientific community.

Please take a second to visit our sponsors' web sites and say thank you for their support of this service.

Accugenix - http://www.accugenix.com

American Type Culture Collection - http://www.atcc.org

BD Diagnostic Systems - http://www.bd.com/ds/

Biolog - http://www.biolog.com

Biomerieux - http://industry.biomerieux-usa.com

Biotest - http://www.BiotestUSA.com/micro

MicroBioLogics, Inc. - http://www.microbiologics.com

Millipore - http://www.millipore.com

MODA Technology Partners: now a LONZA company http://lonza.com/moda

Pall - http://www.pall.com

NovaTek International - http://www.ntint.com

Rapid Micro Biosystems - http://www.rapidmicrobio.com  (formerly GPS)

Veltek Associates, Inc - http://www.sterile.com

=================================
The nature of this service is to provide a medium for communication.  The specific statements and endorsements of individuals participating in the discussions are not necessarily those of The Microbiology Network, Inc., the PMF, or the sponsors of the list.

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