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May 2011


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"Dr. Michael J. Miller" <[log in to unmask]>
Reply To:
The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>
Tue, 17 May 2011 11:52:44 -0400
text/plain (57 lines)
>Hi again Ed. After consideration, PDA may not work. Great for fungi but
>for Bacillus it may be a stretch. I used to work with Bacillus popilliae
>back in my college days (the spores infect Japanese beetle grubs and
>kills them when the spores germinate and cause the blood to turn a milky
>white) and we used a special sporulation medium that, as I can remember,
>included yeast extract and no glucose. It also took weeks to get
>sporulation to occur, hence my earlier recommendation that you may need
>to let the growth go on for some time, regardless of the medium you end
>up using. Good luck! Michael

Michael J. Miller, Ph.D.
Microbiology Consultants, LLC
19009 St. Laurent Drive, Lutz, FL 33558
phone: 727-437-2743 (RAPID-RAPID)
email: [log in to unmask]
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