>Hi again Ed. After consideration, PDA may not work. Great for fungi but
>for Bacillus it may be a stretch. I used to work with Bacillus popilliae
>back in my college days (the spores infect Japanese beetle grubs and
>kills them when the spores germinate and cause the blood to turn a milky
>white) and we used a special sporulation medium that, as I can remember,
>included yeast extract and no glucose. It also took weeks to get
>sporulation to occur, hence my earlier recommendation that you may need
>to let the growth go on for some time, regardless of the medium you end
>up using. Good luck! Michael
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