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July 1999


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Michael C Carroll <[log in to unmask]>
Reply To:
The Pharmaceutical Microbiology Mail List <[log in to unmask]>
Wed, 21 Jul 1999 22:21:36 -0400
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   -A MINIMUM of the organisms in #1, preferably the ones listed in #2,
at 10-100 CFU/vial inoculum; we used vials randomly sampled from the
media fill AFTER the full incubation time (e.g., media that has been
through EVERYTHING that the Media Fill has been through), and the same
incubation as the media fill itself.
        M.Carroll ([log in to unmask])

On Wed, 21 Jul 1999 22:07:09 -0400 Tony & Roz Cundell <[log in to unmask]>
>My collaegues and I wish to ask about the industry practice for
>growth-promotion testing soybean-casein digest broth used in media
>What is the best practice?
>1. Using the USP growth-promotion test for soybean-casein digest broth
>I believe is S. aureus, C. albicans and A. niger incubated at 20-25
>degree C
>for 5 days.
>2. Using an appropriate range of organisms such as B. substilis, S.
>C. albicans and A. niger at the media fill incubation conditions,
>i.e., 7
>days at 20-25 degree C followed by 7 days at 30-35 degree C.
>3. Using say B. substilis, S. aureus incubated at 30-35 degree C for 7
>and C. albicans and A. niger at 20-25 degree C for 7 days.
>Is it OK to use media fill vials for growth-promotion currently with
>media fill incubation or do you need to conduct the the
>test after the 14 days of incubation?
>Tony Cundell
>The PMFList ( is operated from
>The Microbiology Network ( and supported by
>our sponsors ( as a service to
>the scientific community.

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