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Subject:
From:
"Kerrigan, Liz" <[log in to unmask]>
Reply To:
The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>
Date:
Fri, 29 Apr 2011 17:49:18 -0400
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text/plain
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Hi Clint,
It would depend upon the strain and the media that you grow the strain on.
However for most strains, as the colonies age, they appear to spread becoming erose, and irregular.

The type strain of Bacillus clausii, ATCC 700160(TM) is grown on:
ATCC medium 688 is Nutrient agar with pH adjusted to 8.0
Nutrient Agar (BD 213000).........23.0 g
Distilled deionized water..........1.0 L
Autoclave at 121C for 15 minutes. Adjust pH to 8.0.
Conditions:
Temperature:  30C
Atmosphere:   Aerobic
Colonies on medium #688 plates(formulation above) are irregular, rough, glistening, erose to lobate, and low convex.  Colonies appear to have translucent, spreading edges with age.

However strain 221 which is ATCC 21522(TM) is grown on:
ATCC medium #542 Broth:  Peptone-Starch-Carbonate Medium
For solid medium, add 1.5% agar to formula below.
Peptone, 5.0 g
Yeast extract, 5.0 g
K2HPO4, 1.0 g
MgSO4.7H2O, 0.2 g
Soluble starch, 10.0 g
Distilled water, 900.0 ml
Autoclave at 121oC for 15 minutes. 
Cool and add aseptically the following filter-sterilized solution:
10% Na2CO3, 100.0 ml
CONDITIONS: 
Temperature:  37C
Atmosphere:   Aerobic	

On ATCC Medium #542 plates the colonies are pinpoint, translucent and flat with a rough, granular appearance, and an irregular edge.  This strain also grows well on Tryptic Soy Agar (BD 236950).

Both strains described above were isolated from soil.
Hope this helps!
Liz Kerrigan
Director
Standards and Certification
10801 University Boulevard
Manassas, VA 20110-2209
Tel: (703) 365-2813 

-----Original Message-----
From: The Pharmaceutical Microbiology Forum Email List [mailto:[log in to unmask]] On Behalf Of Clint Christensen
Sent: Friday, April 29, 2011 12:07 PM
To: [log in to unmask]
Subject: [PMFLIST] Bacillus clausii

I'm looking for some information about Bacillus clausii  and its colony morphology, specifically the colony size after 5 days incubation.  Is there anyone who is familiar with B. clausii that could help me out with this?

Thanks,
Clint


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The PMFList (http://microbiol.org/PMFList_info.htm) is operated from
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the scientific community.

Please take a second to visit our sponsors' web sites and say thank you for their support of this service.

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American Type Culture Collection - http://www.atcc.org

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MicroBioLogics, Inc. - http://www.microbiologics.com

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Pall - http://www.pall.com

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Rapid Micro Biosystems - http://www.rapidmicrobio.com  (formerly GPS)

Veltek Associates, Inc - http://www.sterile.com

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The nature of this service is to provide a medium for communication.  The specific statements and endorsements of individuals participating in the discussions are not necessarily those of The Microbiology Network, Inc., the PMF, or the sponsors of the list.

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