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Subject:
From:
"Berman, Alison" <[log in to unmask]>
Reply To:
The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>
Date:
Tue, 10 Mar 2020 16:41:26 +0000
Content-Type:
text/plain
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Hello,
You can purchase a flat-bottomed pH probe or you can sacrifice one bottle of media for pH measurement.
If you don't want to sacrifice the media, you can always aseptically pipette out an aliquot into a tube while the media is still liquid and use that instead after it solidifies.


-----Original Message-----
From: The Pharmaceutical Microbiology Forum Email List [mailto:[log in to unmask]] On Behalf Of Ali Raza
Sent: Tuesday, March 10, 2020 8:08 AM
To: [log in to unmask]
Subject: Re: [PMFLIST] Regarding USP 61

[External Email] - This email is originated from outside of the organization. Do not click links or open attachments unless you recognize the sender and know the content is safe

Hi Members;

I have a question regarding culture media pH, in USP (62) states that the pH of different media, if necessary, adjust before sterilization and after sterilization it is within specific medium pH range at 25 °C... My question here is, how we can measure the pH of agar medium at 25 °C whereas it is solidified below 45 °C... How we can use electrode to measure the pH of agar medium at 25 °C...

Kindly guide me in this matter...

Thanks...

-----Original Message-----
From: The Pharmaceutical Microbiology Forum Email List [mailto:[log in to unmask]] On Behalf Of Donald English
Sent: Saturday, March 07, 2020 10:22 PM
To: [log in to unmask]
Subject: Re: [PMFLIST] Regarding USP 61

Dear Sherie,

After reading the section of USP Chapter 61 called Recovery of Microorganisms  in the Presence of of Product,  it states that 1 gram or ml can be used for membrane filtration if you expect high numbers of microorganisms to be present.  In sample preparation for water soluble and non-soluble products, it does states that a 1:10 dilution should be used.  In the section, the amount of sample to be tested is 10 grams or ml’s in which this sample amount is diluted in 90 ml’s of diluent.  If you are conducting membrane filtration of a test sample that contains a high number of microorganisms, I interpreted this read to say that  a 1 gram or ml sample aliquot can be used instead.  In most cases, I would expect that low levels of microorganisms would be present in a test sample.  In addition, I would say that membrane filtration could be used on only those water soluble samples in which there were issues in neutralization of the preservative system by conducting a plate count me!
 thod.

Regards,
Don

Donald J. English Microbiological Quality Consulting LLC Florham Park, New Jersey 07932

Sent from my iPhone

> On Mar 6, 2020, at 3:56 PM, Sherie Poulson-Cook <[log in to unmask]> wrote:
>
> Question for the collective:
>
> In our micro lab we are trying to validate a TVC method for a liquid
> per USP 61. USP 61 specifies under the recovery of microorganisms
> section for membrane filtration that the material filtered should
> represent 1 g of the product. However, under the "Amount Used for the
> Test" section it sates that "unless otherwise directed, use 10 g or 10 mL of the product to be
> examined."   Would we be justified in only filtering 1 mL of product per
> the first statement? \
>
> Regards,
> Sherie
>
>
> Sherie Poulson-Cook
>
>
>
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