A mold may be able to grow in an electrolyte solution due to specific
physiological/metabolic capabilities and not make the transition to grow in
standard fungal growth media.

I agree with our colleagues that staining with lactophenol cotton blue
and/or examining with a wet mount is a good first step in your investigation

On Fri, Feb 24, 2023 at 2:22 PM Shah, Samir <
[log in to unmask]> wrote:

> Hi Megha,
>
> Did you try microscopic evaluation? I have seen few situations in my past
> analyzing complaint sample with "fungal" contamination, which turned out to
> be actual cotton or some fibrous debris floating.
>
> -Samir
>
> -----Original Message-----
> From: The Pharmaceutical Microbiology Forum Email List <
> [log in to unmask]> On Behalf Of Megha Goyal
> Sent: Thursday, February 23, 2023 11:36 PM
> To: [log in to unmask]
> Subject: [PMFLIST] Isolation of stressed fungal cultures from electrolyte
> drink
>
> [Use CAUTION when opening links/attachments]
>
> Dear All,
>
> I work in a product testing company. Recently we received a complaint
> sample of electrolyte solution product (pH around 7) showing white mass as
> contaminant. It is assumed to be a fungal contaminant. We did the necessary
> TYMC using SDA, SCA, PDA plate, even removed the cottony mass and placed it
> on the agar plate. However even on incubating for more than 7 days we did
> not observe any growth.
>
> We assume that since the fungal mass was in high salt solution for a long
> time maybe the fungal growth was stressed and so failed to grow in the
> recommended media. Can anyone suggest how to overcome this? We need to grow
> the fungus to identify it. Is there any media that will help us to recover
> stressed fungus so that it can grow  in the recommended media. Kindly guide
> us.
>
> Thanking you in anticipation,
>
> Regards,
>
> Megha Goyal
>
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