I define it as each sequential time the organism is "spiked" into fresh media and incubated such that a new culture is created. For example: new disk + media + 24 hrs = start culture 1mL start culture + media + 24hrs = 1st pass culture 1mL 1st pass culture + media + 24hrs = 2nd pass culture 1mL 2nd pass culture + media + 24hrs = 3rd pass culture ...............etc Charles Gyecsek [log in to unmask] -----Original Message----- From: Jeanne Wilcox [mailto:[log in to unmask]] Sent: Monday, December 09, 2002 4:06 PM To: [log in to unmask] Subject: [PMFLIST] Culture Passage Guidelines How does the list define "passage" of a microorganism culture? Thanks, Jeanne Wilcox ------------------ The PMFList (http://microbiol.org/PMFList_info.htm) is operated from The Microbiology Network (http://microbiol.org) and supported by our sponsors (http://microbiol.org/sponsor.htm) as a service to the scientific community. Please take a second to visit our sponsors' web sites and say thank you for their support of this service. AAI - http://www.aaiintl.com/Micro.htm Accugenix - http://www.accugenix.com/ Becton Dickinson Microbiology Systems - http://www.bd.com/industrial Chemunex - http://www.chemunex.com/ Microcheck - http://www.microcheck.com/ MIDI - http://www.midi-inc.com/ MIDI Microbial ID - http://www.microbialid.com/ MIDI Labs - http://www.midilabs.com/ Millipore - http://www.millipore.com/ ------------------ The PMFList (http://microbiol.org/PMFList_info.htm) is operated from The Microbiology Network (http://microbiol.org) and supported by our sponsors (http://microbiol.org/sponsor.htm) as a service to the scientific community. Please take a second to visit our sponsors' web sites and say thank you for their support of this service. AAI - http://www.aaiintl.com/Micro.htm Accugenix - http://www.accugenix.com/ Becton Dickinson Microbiology Systems - http://www.bd.com/industrial Chemunex - http://www.chemunex.com/ Microcheck - http://www.microcheck.com/ MIDI - http://www.midi-inc.com/ MIDI Microbial ID - http://www.microbialid.com/ MIDI Labs - http://www.midilabs.com/ Millipore - http://www.millipore.com/
