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December 2002

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Subject:
From:
"Gyecsek, Charles {Envi~Belleville}" <[log in to unmask]>
Reply To:
The Pharmaceutical Microbiology Mail List <[log in to unmask]>
Date:
Tue, 10 Dec 2002 06:57:43 -0500
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I define it as each sequential time the organism is "spiked" into fresh media and incubated such that a new culture is created.  For example:
 
new disk + media + 24 hrs = start culture
1mL start culture + media + 24hrs = 1st pass culture
1mL 1st pass culture + media + 24hrs = 2nd pass culture
1mL 2nd pass culture + media + 24hrs = 3rd pass culture
...............etc
 
Charles Gyecsek
[log in to unmask]
 

-----Original Message-----
From: Jeanne Wilcox [mailto:[log in to unmask]]
Sent: Monday, December 09, 2002 4:06 PM
To: [log in to unmask]
Subject: [PMFLIST] Culture Passage Guidelines



How does the list define "passage" of a microorganism culture? 

Thanks, 
Jeanne Wilcox


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