LISTSERV mailing list manager LISTSERV 16.0

Help for PMFLIST Archives


PMFLIST Archives

PMFLIST Archives


PMFLIST@LISTS.MICROBIOLOGYNETWORK.COM


View:

Message:

[

First

|

Previous

|

Next

|

Last

]

By Topic:

[

First

|

Previous

|

Next

|

Last

]

By Author:

[

First

|

Previous

|

Next

|

Last

]

Font:

Proportional Font

LISTSERV Archives

LISTSERV Archives

PMFLIST Home

PMFLIST Home

PMFLIST  October 2019

PMFLIST October 2019

Subject:

Identification of Isolates Recovered on BCSA

From:

Donald English <[log in to unmask]>

Reply-To:

The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>

Date:

Thu, 3 Oct 2019 13:38:12 -0400

Content-Type:

text/plain

Parts/Attachments:

Parts/Attachments

text/plain (83 lines)

 Dear all,

If you look at the composition of Burkholderia cepacia Selective Agar
(BCSA) that is used in USP Chapter 60, the following ingredients are being
used to inhibit the growth of other microorganisms: Crystal Violet,
Vancomycin, Polymyxin B, and Gentamicin.  Crystal violet has been shown to
inhibit the growth of Gram-positive organisms such as staphylococcus.  Because
of the limitations of Crystal violet in preventing the growth of
enterococci, Vancomycin had been added to the agar formulation to prevent
the growth enterococci.  Polymyxin B and Gentamicin are used to inhibit the
growth of Gram-negative bacilli such as Pseudomonas.  For isolating
Burkholderia cepacia complex from sputum samples, it is important to have
these inhibitory agents due to the presence of other organisms.

However, it should be realized that BCSA is not 100% effective in
preventing the isolation of non-Burkholderia cepacia complex organisms from
a test sample.  If you look at the 1999 paper by Henry et al. (Reference:
Henry, D, Campbell, M., McGimpsey, C., Clarke, A., Louden, L. Burns, JL,
Roe, MH, Vandamme, P. and Speert, D. 1999.  Comparison of Isolation Media
for Recovery of Burkholderia cepacia complex for respiratory secretions of
patients with cystic fibrous. J. Clin. Microbiol. 37: 1004-1007), they were
able to isolate the following microbial species on BCSA:  Burkholderia
gladioli, Ralsontia pickettii, Achromobacter xylosoxidans, and
Chryseobacterium (Flavobacterium) indologenes.  If you are not using
16srRNA or MALDI-TOF analysis for conducting identification of
microorganisms, Burkholderia gladioli and Ralsontia pickettii are somewhat
difficult to separate biochemically from Burkholderia cepacia complex and
require additional biochemical testing.  For example, Burkholderia gladioli
can be separated from Burkholderia cepacia complex by having a positive
reaction for the oxidation of Maltose and Lactose.  Chryseobacterium
indologenes has a positive indole reaction that can be used to separate it
from Burkholderia cepacia complex.  I have also heard of reports that
Enterococcus faecalis and Stenotrophomonas maltophilia (colistin resistant
strains) can also be isolated from a test sample by using BCSA, but I do
not have a literature reference.

If you are going to perform microbial testing of aqueous samples by using
USP Chapter 60 in which Burkholderia cepacia Selective Agar is used, it is
important not to rely on the presumptive identification of isolates on this
agar due to the possible recovery of non-Burkholderia cepacia complex
species as indicated above.  Instead, I would recommend that any isolate
recovered on BCSA be identified to the species level by using 16s rRNA or
MALDI-TOF analysis.  If those laboratories that do not have gene sequencer
or a MALDI-TOF for conducting identifications of microbial isolates,
additional biochemical tests will need to be used for conducting
biochemical identification of the recovered isolate to confirm the
identification.

 Don

 Donald J. English Microbiological Quality Consulting LLC

Florham Park, New Jersey 07932

------------------
The PMFList (http://microbiologynetwork.com/pmflist.asp) is operated from
The Microbiology Network (http://microbiologynetwork.com) and supported by
our sponsors as a service to the scientific community.

Please take a second to visit our sponsors' web sites and say thank you for their support of this service.
If your company would be interested in sponsoring this community, please contact [log in to unmask]



Science Advisory Board https://www.scienceboard.net/

Steris - http://www.sterislifesciences.com/

Charles River Laboratories - http://www.criver.com/

Veltek Associates, Inc - http://www.sterile.com

Microbiologics, Inc. - http://www.microbiologics.com

BD Industrial Media - http://www.bd.com/ds/

Boston Analytical http://www.bostonanalytical.com/

Associates of Cape Cod, Inc. - http://www.acciusa.com/


=================================
The nature of this service is to provide a medium for communication.  The specific statements and endorsements of individuals participating in the discussions are not necessarily those of The Microbiology Network, Inc., the PMF, or the sponsors of the list.

Top of Message | Previous Page | Permalink

Advanced Options


Options

Log In

Log In

Get Password

Get Password


Search Archives

Search Archives


Subscribe or Unsubscribe

Subscribe or Unsubscribe


Archives

October 2019
September 2019
August 2019
July 2019
June 2019
May 2019
April 2019
March 2019
February 2019
January 2019
December 2018
November 2018
October 2018
September 2018
August 2018
July 2018
June 2018
May 2018
April 2018
March 2018
February 2018
January 2018
December 2017
November 2017
October 2017
September 2017
August 2017
July 2017
June 2017
May 2017
April 2017
March 2017
February 2017
January 2017
December 2016
November 2016
October 2016
September 2016
August 2016
July 2016
June 2016
May 2016
April 2016
March 2016
February 2016
January 2016
December 2015
November 2015
October 2015
September 2015
August 2015
July 2015
June 2015
May 2015
April 2015
March 2015
February 2015
January 2015
December 2014
November 2014
October 2014
September 2014
August 2014
July 2014
June 2014
May 2014
April 2014
March 2014
February 2014
January 2014
December 2013
November 2013
October 2013
September 2013
August 2013
July 2013
June 2013
May 2013
April 2013
March 2013
February 2013
January 2013
December 2012
November 2012
October 2012
September 2012
August 2012
July 2012
June 2012
May 2012
April 2012
March 2012
February 2012
January 2012
December 2011
November 2011
October 2011
September 2011
August 2011
July 2011
June 2011
May 2011
April 2011
March 2011
February 2011
January 2011
December 2010
November 2010
October 2010
September 2010
August 2010
July 2010
June 2010
May 2010
April 2010
March 2010
February 2010
January 2010
December 2009
November 2009
October 2009
September 2009
August 2009
July 2009
June 2009
May 2009
April 2009
March 2009
February 2009
January 2009
December 2008
November 2008
October 2008
September 2008
August 2008
July 2008
June 2008
May 2008
April 2008
March 2008
February 2008
January 2008
December 2007
November 2007
October 2007
September 2007
August 2007
July 2007
June 2007
May 2007
April 2007
March 2007
February 2007
January 2007
December 2006
November 2006
October 2006
September 2006
August 2006
July 2006
June 2006
May 2006
April 2006
March 2006
February 2006
January 2006
December 2005
November 2005
October 2005
September 2005
August 2005
July 2005
June 2005
May 2005
April 2005
March 2005
February 2005
January 2005
December 2004
November 2004
October 2004
September 2004
August 2004
July 2004
June 2004
May 2004
April 2004
March 2004
February 2004
January 2004
December 2003
November 2003
October 2003
September 2003
August 2003
July 2003
June 2003
May 2003
April 2003
March 2003
February 2003
January 2003
December 2002
November 2002
October 2002
September 2002
August 2002
July 2002
June 2002
May 2002
April 2002
March 2002
February 2002
January 2002
December 2001
November 2001
October 2001
September 2001
August 2001
July 2001
June 2001
May 2001
April 2001
March 2001
February 2001
January 2001
December 2000
November 2000
October 2000
September 2000
August 2000
July 2000
June 2000
May 2000
April 2000
March 2000
February 2000
January 2000
December 1999
November 1999
October 1999
September 1999
August 1999
July 1999
June 1999
May 1999
April 1999
March 1999
February 1999
January 1999
December 1998
November 1998
October 1998
September 1998
August 1998
July 1998
June 1998
May 1998
April 1998
March 1998
February 1998
January 1998

ATOM RSS1 RSS2



LISTS.MICROBIOLOGYNETWORK.COM

CataList Email List Search Powered by the LISTSERV Email List Manager