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May 2011

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Subject:
From:
"Steven G. Richter" <[log in to unmask]>
Reply To:
The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>
Date:
Tue, 17 May 2011 00:43:20 +0000
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Hi Ed

Did you add Maganese sulfate to the media?

Steve's phone

On May 16, 2011, at 7:32 PM, "Dr. Michael J. Miller" <[log in to unmask]> wrote:

> Hi Ed. Have you tried growing on Potato Dextrose Agar? You may also need
> to incubate for a longer time period. Michael
> 
> Michael J. Miller, Ph.D.
> President
> Microbiology Consultants, LLC
> 19009 St. Laurent Drive, Lutz, FL 33558
> phone: 727-437-2743 (RAPID-RAPID)
> email: [log in to unmask]
> web: http://microbiologyconsultants.com
> linkedIn: http://www.linkedin.com/in/drmichaelmiller
> 
> http://rapidmicromethods.com  advancing the science and implementation of
> rapid microbiological methods.
> 
> 
> 
> On 5/11/11 9:05 AM, "Balkovic, Ed" <[log in to unmask]> wrote:
> 
>> We are working with Bacillus sphaericus and trying to make a spore
>> suspension.  We are having issues getting it to sporulate.  We have
>> tried sporulation agar and even placing the plates after growth in the
>> refrigerator for a week.
>> 
>> Anybody have experience with this bug and any suggestions you might have
>> for us. 
>> 
>> Thanks in advance,
>> 
>> Ed Balkovic, Ph.D.
>> Quality Control Technical Services
>> Genzyme Corporation
>> [log in to unmask]
>> 
>> The opinions expressed above are my own and may not reflect those of my
>> employer. 
>> 
>> 
>> 
>> 
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> ------------------
> The PMFList (http://microbiol.org/PMFList_info.htm) is operated from
> The Microbiology Network (http://microbiol.org) and supported by
> our sponsors (http://microbiol.org/sponsor.htm) as a service to
> the scientific community.
> 
> Please take a second to visit our sponsors' web sites and say thank you for their support of this service.
> 
> Accugenix - http://www.accugenix.com
> 
> American Type Culture Collection - http://www.atcc.org
> 
> BD Diagnostic Systems - http://www.bd.com/ds/
> 
> Biolog - http://www.biolog.com
> 
> Biomerieux - http://industry.biomerieux-usa.com
> 
> Biotest - http://www.BiotestUSA.com/micro
> 
> MicroBioLogics, Inc. - http://www.microbiologics.com
> 
> MODA Technology Partners: now a LONZA company http://lonza.com/moda
> 
> Pall - http://www.pall.com
> 
> NovaTek International - http://www.ntint.com
> 
> Rapid Micro Biosystems - http://www.rapidmicrobio.com  (formerly GPS)
> 
> Veltek Associates, Inc - http://www.sterile.com
> 
> =================================
> The nature of this service is to provide a medium for communication.  The specific statements and endorsements of individuals participating in the discussions are not necessarily those of The Microbiology Network, Inc., the PMF, or the sponsors of the list.

------------------
The PMFList (http://microbiol.org/PMFList_info.htm) is operated from
The Microbiology Network (http://microbiol.org) and supported by
our sponsors (http://microbiol.org/sponsor.htm) as a service to
the scientific community.

Please take a second to visit our sponsors' web sites and say thank you for their support of this service.

Accugenix - http://www.accugenix.com

American Type Culture Collection - http://www.atcc.org

BD Diagnostic Systems - http://www.bd.com/ds/

Biolog - http://www.biolog.com

Biomerieux - http://industry.biomerieux-usa.com

Biotest - http://www.BiotestUSA.com/micro

MicroBioLogics, Inc. - http://www.microbiologics.com

MODA Technology Partners: now a LONZA company http://lonza.com/moda

Pall - http://www.pall.com

NovaTek International - http://www.ntint.com

Rapid Micro Biosystems - http://www.rapidmicrobio.com  (formerly GPS)

Veltek Associates, Inc - http://www.sterile.com

=================================
The nature of this service is to provide a medium for communication.  The specific statements and endorsements of individuals participating in the discussions are not necessarily those of The Microbiology Network, Inc., the PMF, or the sponsors of the list.

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