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The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>
Date:
Fri, 18 Dec 2015 17:12:48 +0530
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If you have got nil count/zero for 10ml filtration, i think result should
be <10cfu/gm.

Thanks and Regards
Jalis Muktadir
[log in to unmask]
9967534796
On 17 Dec 2015 8:17 am, "Elizabeta Popovska" <[log in to unmask]> wrote:

> Dear members,
>
> Do you think this is a good way to express the results in membrane
> filtration method?
>
>
> “We test microbial count according to Ph Eur. 2.6.12 with Membrane
> filtration:
>
> Test solution: we weighed 10g of xxxxxxxx   dissolve and diluted to 100ml
> with diluent (PH 7.0 sodium chloride-peptone buffer solution).
>
> Then, we transfer 10ml of test solution and slowly added into the
> sterilizing filter and filter immediately. Wash each filter with three
> times, each time for 100ml of diluent (0.1% peptone water). That's mean
> actually 1g of the xxxxxxx  is used to test microbial count, which is
> correspondence with Ph Eur. 2.6.12
> So, based on this, even if we use a dilution 1:10, actually it is 1g
> of xxxxxxxxxxxxxx  as we transfer 10ml of test solution to filter.
>
> So the results should be <1CFU/g or 1CFU/g.”
>
>
> Regards
>
> Elizabeta
>
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our sponsors as a service to the scientific community.

Please take a second to visit our sponsors' web sites and say thank you for their support of this service.

Veltek Associates, Inc - http://www.sterile.com

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Analytical Research Labs - http://arlok.com/

Life Technologies - http://www.lifetechnologies.com/PharmaAnalytics

Charles River-Endosafe and Accugenix - http://www.criver.com/emd

American Type Culture Collection - http://www.atcc.org

ATS Labs - http://www.ats-labs.com

BD Diagnostic Systems - http://www.bd.com/ds/

Biolog - http://www.biolog.com

EMD Millipore -  http://www.emdmillipore.com/biomonitoring

Microbiologics, Inc. - http://www.microbiologics.com

Microtest Laboratories, Inc. - http://www.microtestlabs.com

Nelson Labs - http://www.nelsonlabs.com

Steris - http://www.sterislifesciences.com/



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