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Date: | Thu, 22 Jul 1999 08:31:00 +0100 |
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Tony,
We perform growth promotion tests on media before use, and after the 14 days
broth incubation.
Tim Sandle, BPL
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From: Tony & Roz Cundell
To: TS20365; 'PMFLIST@SMTP <[log in to unmask]>'
Subject: [PMFLIST] Growth Promotion Testing Media
Date: 21 July 1999 22:07
Original Subject:
[PMFLIST] Growth Promotion Testing Media Fill Material
My collaegues and I wish to ask about the industry practice for
growth-promotion testing soybean-casein digest broth used in media fills.
What is the best practice?
1. Using the USP growth-promotion test for soybean-casein digest broth which
I believe is S. aureus, C. albicans and A. niger incubated at 20-25 degree C
for 5 days.
2. Using an appropriate range of organisms such as B. substilis, S. aureus,
C. albicans and A. niger at the media fill incubation conditions, i.e., 7
days at 20-25 degree C followed by 7 days at 30-35 degree C.
3. Using say B. substilis, S. aureus incubated at 30-35 degree C for 7 days
and C. albicans and A. niger at 20-25 degree C for 7 days.
Is it OK to use media fill vials for growth-promotion currently with the
media fill incubation or do you need to conduct the the growth-promotion
test after the 14 days of incubation?
Tony Cundell
Wyeth-Ayerst
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