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February 2020

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Subject:
From:
Timothy Wood <[log in to unmask]>
Reply To:
The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>
Date:
Fri, 31 Jan 2020 13:39:08 -0800
Content-Type:
text/plain
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text/plain (103 lines)
Barry,

In addition to Tony Cundell's work, Tim Sandle did work using R3A less
nutrient media for pharmaceutical water.  It stated: "the R3A recovered B.
cepacia satisfactorily"

https://www.researchgate.net/publication/267332592_Assessment_of_the_suitabi
lity_of_R3A_agar_for_the_subculture_of_microorganisms_isolated_from_pharmace
utical_water_systems/link/544bfca70cf24b5d6c409234/download

Timothy D. Wood
President and Principal Consultant
Cell Therapy Microbiology Consulting, LLC
9865 Jackman Rd.
Lynden, WA 98264
Cell #: 425-281-8750
www.celltherapymicro.com


-----Original Message-----
From: The Pharmaceutical Microbiology Forum Email List On Behalf Of
Mcnamara, Barry
Sent: Friday, January 31, 2020 8:02 AM
To: [log in to unmask]
Subject: [PMFLIST] R2A Plate Media Incubation Guidance for Optimal Recovery
of BCC from Water

All:

I am seeking additional references/clarification for incubation temp and
time for optimal recoveries of BCC orgs from manufacturing bulk water
samples (if present) when using R2A plate media in conjunction with membrane
filtration only. EP monograph for Water, Purified .0008 describes a minimal
incubation time of 3 days at 30-35C whereas guidance from Standard Method
for Examination of water/wastewater 9213E recommends incubation at 35C for
minimum 48 h but with option for 20-28C for 5-7 days for optimal recovery
(i.e. highest quantitative counts for HPCs in general). Which of these two
options should be applied for use in recovery specifically for BCC orgs?

Barry P. McNamara, Ph.D.


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------------------
The PMFList (http://microbiologynetwork.com/pmflist.asp) is operated from
The Microbiology Network (http://microbiologynetwork.com) and supported by
our sponsors as a service to the scientific community.

Please take a second to visit our sponsors' web sites and say thank you for their support of this service.
If your company would be interested in sponsoring this community, please contact [log in to unmask]



Steris - http://www.sterislifesciences.com/

Charles River Laboratories - http://www.criver.com/

Veltek Associates, Inc - http://www.sterile.com

Microbiologics, Inc. - http://www.microbiologics.com

BD Industrial Media - http://www.bd.com/ds/

Boston Analytical http://www.bostonanalytical.com/

Associates of Cape Cod, Inc. - http://www.acciusa.com/


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The nature of this service is to provide a medium for communication.  The specific statements and endorsements of individuals participating in the discussions are not necessarily those of The Microbiology Network, Inc., the PMF, or the sponsors of the list.

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