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June 2020

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Subject:
From:
"Shah, Samir" <[log in to unmask]>
Reply To:
The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>
Date:
Tue, 23 Jun 2020 19:16:23 +0000
Content-Type:
text/plain
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Dear all,

There are three issues we are dealing with here- segregation, cross contamination and security. While having dedicated "clean" and "dirty" refrigerators are ideal from the lab design and flow point of view, it's not practical and economical for each lab. Microorganisms are not going to jump from plates and slants or tubes to sterile media. Even the theory of top shelf versus bottom shelf does not sit right in my mind but if it helps with segregation, so be it. Clear segregation in baskets or closed containers and label is enough for GMP purpose. Think twice before making permanent labels on walls or shelves. I have seen working culture tubes on shelves marked as Culture Media and vice versa. Cleaning and sanitization frequency including the cooling fan cleaning is critical element of periodic maintenance and your best bet in avoiding gross aerial contaminations inside incubators and refrigerators.

If security is a concern- card key access to the lab, user log, locked doors with key pad access, etc. are various measures. Although I must say, never heard of padlock on a container in my 30 years in the industry. It just increases the time you have to spend in a walk in chamber and might cause more problem.

-Samir

Disclaimer: The personal opinion presented above is solely based on my education and experience.


-----Original Message-----
From: The Pharmaceutical Microbiology Forum Email List <[log in to unmask]> On Behalf Of Vishwas Kelkar
Sent: Tuesday, June 23, 2020 11:23 AM
To: [log in to unmask]
Subject: Re: [PMFLIST] Storage of cultures and media

[Use CAUTION when opening links/attachments]

Hello,
I have seen people storing the microbiological cultures, including new vials, inside locked containers with numbered padlocks and the in-use cultures separately inside a locked container with a combination lock, along with prepared media in the same walk-in refrigerated rooms maintained at 2-8 degC.

Thanks,
Vishwas



-----Original Message-----
From: The Pharmaceutical Microbiology Forum Email List <[log in to unmask]> On Behalf Of Tony Cundell
Sent: Monday, June 22, 2020 2:17 PM
To: [log in to unmask]
Subject: Re: [PMFLIST] Storage of cultures and media

Shweta,

Although it may look better to segregate live cultures and media, in labs I have managed they have been successfully stored on separate in walk-in refrigerated rooms under the conditions you describe

Tony.

On Mon, Jun 22, 2020 at 2:09 PM Shweta Bajoria <[log in to unmask]> wrote:

> Hello experts,
> What is the industry practise for storage of media and cultures together.
> We are storing prepared media for 15 days in a cooling chamber 2-8
> degrees and store working cultures on nutrient media in separate
> refrigerator . Can they be stored together in separate trays in one equipment.
> All media is wrrapped and all cultures remain intact in close test tubes.
>
> Regards
> Dr. Shweta Mittal
>
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--
Tony Cundell, Ph. D.
Consulting Microbiologist
Email: [log in to unmask]
Phone: 914 725-3947
Cell: 914 841-0074

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