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April 2000

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The Pharmaceutical Microbiology Mail List <[log in to unmask]>
Date:
Thu, 27 Apr 2000 15:43:21 -0500
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This sounds like an interesting organism.
You might want to send this down to Dr. Rinaldi's lab in Texas for the
identification work; he is pretty much The Man when it comes to fungal ID's.
Sorry I don't have a phone number handy, but here's the address:
 Medical Mycology Research Center
Department of Pathology
University of Texas Medical Branch
Galveston, Texas 77555-0609

Would you happen to have any photos of the gross colony morphology and the
microscopic lactophenol aniline blue mounts that you could post here?   Good
luck on finding answers to your problem organism!
Pam Sturm

Aventis Pharmaceutical Inc.
Sr. Associate Microbiologist
Mail: C1-M0619
Phone: (816) 966-5668
e-mail: [log in to unmask] <mailto:[log in to unmask]>



-----Original Message-----
From: mcc [mailto:[log in to unmask]]
Sent: Thursday, April 27, 2000 9:00 AM
To: [log in to unmask]
Subject: [PMFLIST] Thermophilic Mold


THIS is a real, genuine cry for help.
During revalidation studies of sterilization processes, we have begun
encountering positives, which are NOT B. stearothermophilus or any other
bacteria-- they are a MOLD, source and type unknown. This thing grows ONLY
at elevated temperatures (55 C), and best on TSA (much more slowly and
poorly on SAB). Visually, it appears whitish and without aerial mycelia;
microscopically, it is small, and resembles either Rhizomucor or Actinomyces
(colonies look more like Actinomyces).
SEVERAL QUESTIONS:
1) Who, in reference labs, academia, or industry, is equipped to do work on
such organisms, such as identification and/or "D-value" studies?
2) Is there any body of literature and/or scientific forum out there, which
would support any claim that a sterilization process, aimed at generating
Fo's of 24 and above, would be adequate to obliterate such an organism and
its spores?
   TIME is of the essence-- all replies and assistance gratefully
appreciated. THANKS in advance--
  M.Carroll ([log in to unmask])


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