PMFLIST Archives

December 2003


Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Cindy Duhigg <[log in to unmask]>
Reply To:
The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>
Wed, 17 Dec 2003 10:13:36 EST
text/plain (54 lines)
The "Glassware Cleaning" section of any of Anatel's stock SOP's describes a
laboratory detergent/hydrogen peroxide cleaning sequence that works very well
in practical use.  Anatel also recommends chromic acid for recalcitrant items
(yeah, I know - when there's a better alternative I'll use it, too), and I have
always required this step for new glassware or glassware with uncertain

Good document source is:, and a good SOP example is available
at, "Glassware Cleaning".

NOW; once those flasks are clean, DEDICATE THEM, and store them carefully
(covering the mouth of each flask with foil helps)!  It is much easier than
pursuing a failure investigation every time someone from the QC lab borrows a piece
of glassware and cleans it to "laboratory standards".

Good luck,
Cindy Duhigg

In a message dated 12/17/2003 8:53:26 AM Central Standard Time,
[log in to unmask] writes:

> Can anyone tell me what is the best way to clean glassware for TOC?  I am
> used to just buying the glassware and I guess never asked how they cleaned
> it.  Currently they depyrogenate the TOC glassware here and I was unsure
> if that was good enough.
> Any help would be greatly appreciated.
> Gayle Shaw
> QE Validation Microbiologist
> Cryolife, Inc

The PMFList ( is operated from
The Microbiology Network ( and supported by
our sponsors ( as a service to
the scientific community.

Please take a second to visit our sponsors' web sites and say thank you for their support of this service.
Accugenix -
Becton Dickinson Microbiology Systems -
Microcheck -
MIDI, Inc. -
Microbial ID, Inc. -
MicroTest World Class Services -
MIDI Labs, Inc. -
Millipore -
NovaTek International -
Raven Biological Labs -
Veltek Associates, Inc -