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December 2003

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Subject:
From:
"Booth, Crystal" <[log in to unmask]>
Reply To:
The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>
Date:
Thu, 18 Dec 2003 10:36:38 -0500
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Gayle,

We use Standard Method's Agar for Total Count, and Colilert for
presence/absence of Coliforms.

Colilert is easy to use.  Just add a bubble pack to a 100 mL sample and
incubate it for 24 hours.  If there is a color change, Coliforms are
present.  Hold the sample under a UV light in a dark location, if the sample
fluoresces, E. coli is present.  Check out www.idexx.com for more
information on Colilert and other great water products.

Regards,
Crystal Booth
Microbiologist

-----Original Message-----
From: Gayle Shaw [mailto:[log in to unmask]]
Sent: Tuesday, December 16, 2003 4:13 PM
To: [log in to unmask]
Subject: [PMFLIST] question on media used for Water Bioburden testing


Hello everyone,
Last question for the week I hope:
What is everyone using for media to test their water?  Are you using just
one type (R2A, HPC, or TSB) or are you using Mendo for coliform, R2A or
similar for total count and Cetrimide or something similar for Pseudomonas
species?

Thanks for your replies

Gayle Shaw
QE Validation Microbiologist
Cryolife, Inc




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The PMFList (http://microbiol.org/PMFList_info.htm) is operated from
The Microbiology Network (http://microbiol.org) and supported by
our sponsors (http://microbiol.org/sponsor.htm) as a service to
the scientific community.

Please take a second to visit our sponsors' web sites and say thank you for their support of this service.
AAI - http://www.aaiintl.com/Micro.htm
Accugenix - http://www.accugenix.com/
Becton Dickinson Microbiology Systems - http://www.bd.com/industrial
Microcheck - http://www.microcheck.com/
MIDI, Inc. - http://www.midi-inc.com/
Microbial ID, Inc. - http://www.microbialid.com/
MicroTest World Class Services - http://www.microtestlabs.com/
MIDI Labs, Inc. - http://www.midilabs.com/
Millipore - http://www.millipore.com/
NovaTek International - http://www.ntint.com
Raven Biological Labs - http://www.ravenlabs.com
Veltek Associates, Inc - http://www.sterile.com

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