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December 2003

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Subject:
From:
Phil Geis <[log in to unmask]>
Reply To:
The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>
Date:
Fri, 12 Dec 2003 12:54:19 -0500
Content-Type:
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Establishing "stability" in house presumes manufacturer will control and
maintain as constant all stain reagent parameters significant to stability.  It
also presumes that you are aware of all critical parameters that need to be
examined. Maybe these are reasonable to presume - still I suggest you contact
manuf 1st to get their perspective.

Also, I'm not so familiar with using Copland jars for Gram staining but haven't
the volume of staining the would require it.  Unless you have a large number of
Gram stains to perform, you might be better off getting prepared reagents,
maintaining in the packages supplied and using the manufacturer's stability
data.




From: "U. G. Barad (M. Pharm., Ph. D.)" <[log in to unmask]> on 12/12/2003
03:15 PM GMT

   "U. G. Barad (M. Pharm., Ph.           To:   [log in to unmask]
   D.)" <[log in to unmask]>           Cc:    (bcc: Phil Geis-PA/PGI)
                                  Subject:      Re: [PMFLIST] Gram Staining

            12/12/2003 10:15 AM
          Please respond to The
    Pharmaceutical Microbiology
               Forum Email List
 <[log in to unmask]>






Hi Sarah,

I appreciated your concern. But your message is not very clear, as you have
not specified the manufacturer's name for gram stain reagent you have been
using. The best way out is do your own study specific to the manufacturer.
And accordingly fix up the expiration dating for that manufacturer and for
the environment under which you are using the reagent. Best regards,

U. G. Barad (M. Pharm., Ph. D.)
Sr. Q. A. Manager
[log in to unmask]






----- Original Message -----
From: "Sarah Price" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Friday, December 12, 2003 9:17 AM
Subject: [PMFLIST] Gram Staining


> When dispensing gram stain reagents from a manufacturer's bottle to a
Coplin
> Staining dish what expiration should be given once in the dish?  Any
> suggestions?
> The dish will be covered and wrapped around with parafilm to prevent
> evaporation of the reagents. Thanks for your help.
>
> Sarah
>
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Accugenix - http://www.accugenix.com/
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Microcheck - http://www.microcheck.com/
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MicroTest World Class Services - http://www.microtestlabs.com/
MIDI Labs, Inc. - http://www.midilabs.com/
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Veltek Associates, Inc - http://www.sterile.com

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The PMFList (http://microbiol.org/PMFList_info.htm) is operated from
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our sponsors (http://microbiol.org/sponsor.htm) as a service to
the scientific community.

Please take a second to visit our sponsors' web sites and say thank you for their support of this service.
AAI - http://www.aaiintl.com/Micro.htm
Accugenix - http://www.accugenix.com/
Becton Dickinson Microbiology Systems - http://www.bd.com/industrial
Microcheck - http://www.microcheck.com/
MIDI, Inc. - http://www.midi-inc.com/
Microbial ID, Inc. - http://www.microbialid.com/
MicroTest World Class Services - http://www.microtestlabs.com/
MIDI Labs, Inc. - http://www.midilabs.com/
Millipore - http://www.millipore.com/
NovaTek International - http://www.ntint.com
Raven Biological Labs - http://www.ravenlabs.com
Veltek Associates, Inc - http://www.sterile.com

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