PMFLIST Archives

October 2005


Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Manasith <[log in to unmask]>
Reply To:
The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>
Mon, 24 Oct 2005 13:16:29 +0300
text/plain (69 lines)
Dear colleague!

We are manufacturer of pharmaceutical non-sterile products. I would like
to ask the forum about the environmental monitoring in the Penicillin
and Cephalosporin units.


The question is: which kind of medium should be used for the detection
of bacteria? 

We use the air sampler for collection of air on Tryptone soya agar

Do we have to use medium, which contain neutralizers? We used to compare
the recovery rate between the normal TSA with TSA containing
Tween/Lecithin and TSA containing penicillinase.

As results, we could not find any significant difference in the recovery
between 3 agar media, maybe due to the low amount of antibiotic in the
air? Or the neutralizer is not effective enough?


Has some one experience in this field or can some one tell me which
medium should I use? Is the use of general neutralizers Tween and
Lecithin enough for the environmental monitoring in these units?

Should I measure the concentration of penicillin in the air and
calculate the amount of Penicillinase to be put in the medium? I think,
the concentration of penicillin in the air should very with the time.


Thanks you,


Manasith Phithak

Microbiology laboratory

The PMFList ( is operated from
The Microbiology Network ( and supported by
our sponsors ( as a service to
the scientific community.

Please take a second to visit our sponsors' web sites and say thank you for their support of this service.

Accugenix -
ATS Laboratories -
Biolog -
Biomerieux -
Dupont Qualicon -
EMSL Analytical, Inc. -
Genomic Profiling Systems, Inc. -
Microcheck Inc,  Microbial Analysis Laboratory -
MicroTest World Class Services -
MIDI, Inc. -
Millipore -
NovaTek International -
Raven Biological Labs -
Veltek Associates, Inc -

The nature of this service is to provide a medium for communication.  The specific statements and endorsements of individuals participating in the discussions are not necessarily those of The Microbiology Network, Inc., the PMF, or the sponsors of the list.