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May 2006

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The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>
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Thu, 4 May 2006 07:28:43 +1200
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Curtis
Following on from Tony's advice I suggest that you use a different solvent.
Given that polysorbate and lecithin may only add to your troubles an
alternative diluent/solvent is Isopropyl myristrate*. (IPM) If you use IPM
you can use a filtration method. Dissolve the oil in IPM and filter once
fully filtered you may then add aqueous diluents to rinse the filter.
Points to note:
 Do not sterilise the IPM by heating as the pH will drop to about 4.5.
(Filter sterilise)
Filter to use is Millipore Durapore or equivalent (Cellulose filters are
incompatable)
Avoid use of some plastics as IPM has a solvent action (IPM tends to attack
the plastic in petri dishes)
*Also known as Myristic acid isopropyl ester (Sigma)

Brendan Lincoln




                                                                           
             Tony Cundell                                                  
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             <[log in to unmask]         Re: [PMFLIST] Soybean oil           
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             04/05/06 02:03                                                
                                                                           
                                                                           
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Curtis,

Soybean oil is bacteriostatic due to the presence of fatty acids so the
strategy of using polysorbate 20 and Lecithin as neutralizers and modest
dilution probably will not work.

The method of maufacture is solvent extraction using petroluem hydrocarbons
from crushed soybeans, filtration at 0 degree C, and alkali treatment to
removal sterols and phospolipids.

The maunacturing process and low water activity does not make the material
a
risk for microbial contamination



Tony Cundell
Scarsdale, NY





>From: "merideth, curtis" <[log in to unmask]>
>Reply-To: The Pharmaceutical Microbiology Forum Email List
><[log in to unmask]>
>To: [log in to unmask]
>Subject: [PMFLIST] Soybean oil inhibition
>Date: Tue, 2 May 2006 09:59:08 -0500
>
>Does anyone know if soybean oil has inhibitory properties, and if so how
>to overcome them for USP total aerobic count?
>
>I've tried diluting up to 1:100 in SCD broth with polysorbate and
>lecithin with no luck for recovery of S. aureus.  A dilution of 1:20
>gave the same result as 1:100, about 30% recovery of S. aureus.  The
>product is a capsule that dissolves almost completely if the broth is
>warmed, but I don't think it would be "suitable" for membrane
>filtration.  I could try filtering a portion of the broth or multiple
>tube method if I needed to, but I'd like to avoid that if I could.  Any
>other ideas?  Or do you think it would be safe to just monitor at 1:20
>or 1:100?  Every other organism recovered fine at these dilutions.  Any
>help would be appreciated.
>
>Curt Merideth
>Microbiologist
>
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Please take a second to visit our sponsors' web sites and say thank you for their support of this service.

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EMD Chemicals - http://www.emdchemicals.com

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Genomic Profiling Systems, Inc. - http://www.genprosys.com

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Millipore - http://www.millipore.com

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Raven Biological Labs - http://www.ravenlabs.com

Veltek Associates, Inc - http://www.sterile.com

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