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May 2006

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Subject:
From:
Tony Cundell <[log in to unmask]>
Reply To:
The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>
Date:
Fri, 5 May 2006 08:36:48 -0400
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Brendan,

Good suggestion. IPM has a long history of use in MF sterility tests.
I still question whether it would be necessary to routinely test soybean 
oil.

Tony Cundell
Scarsdale, NY





>From: [log in to unmask]
>Reply-To: The Pharmaceutical Microbiology Forum Email List              
><[log in to unmask]>
>To: [log in to unmask]
>Subject: Re: [PMFLIST] Soybean oil inhibition
>Date: Thu, 4 May 2006 07:28:43 +1200
>
>Curtis
>Following on from Tony's advice I suggest that you use a different solvent.
>Given that polysorbate and lecithin may only add to your troubles an
>alternative diluent/solvent is Isopropyl myristrate*. (IPM) If you use IPM
>you can use a filtration method. Dissolve the oil in IPM and filter once
>fully filtered you may then add aqueous diluents to rinse the filter.
>Points to note:
>  Do not sterilise the IPM by heating as the pH will drop to about 4.5.
>(Filter sterilise)
>Filter to use is Millipore Durapore or equivalent (Cellulose filters are
>incompatable)
>Avoid use of some plastics as IPM has a solvent action (IPM tends to attack
>the plastic in petri dishes)
>*Also known as Myristic acid isopropyl ester (Sigma)
>
>Brendan Lincoln
>
>
>
>
>
>              Tony Cundell
>              <[log in to unmask]
>              >                                                          To
>              Sent by: The              [log in to unmask]
>              Pharmaceutical                                             cc
>              Microbiology
>              Forum Email List                                      Subject
>              <[log in to unmask]         Re: [PMFLIST] Soybean oil
>              CROBIOL.ORG>              inhibition
>
>
>              04/05/06 02:03
>
>
>              Please respond to
>                     The
>               Pharmaceutical
>                Microbiology
>              Forum Email List
>              <[log in to unmask]
>                CROBIOL.ORG>
>
>
>
>
>
>
>Curtis,
>
>Soybean oil is bacteriostatic due to the presence of fatty acids so the
>strategy of using polysorbate 20 and Lecithin as neutralizers and modest
>dilution probably will not work.
>
>The method of maufacture is solvent extraction using petroluem hydrocarbons
>from crushed soybeans, filtration at 0 degree C, and alkali treatment to
>removal sterols and phospolipids.
>
>The maunacturing process and low water activity does not make the material
>a
>risk for microbial contamination
>
>
>
>Tony Cundell
>Scarsdale, NY
>
>
>
>
>
> >From: "merideth, curtis" <[log in to unmask]>
> >Reply-To: The Pharmaceutical Microbiology Forum Email List
> ><[log in to unmask]>
> >To: [log in to unmask]
> >Subject: [PMFLIST] Soybean oil inhibition
> >Date: Tue, 2 May 2006 09:59:08 -0500
> >
> >Does anyone know if soybean oil has inhibitory properties, and if so how
> >to overcome them for USP total aerobic count?
> >
> >I've tried diluting up to 1:100 in SCD broth with polysorbate and
> >lecithin with no luck for recovery of S. aureus.  A dilution of 1:20
> >gave the same result as 1:100, about 30% recovery of S. aureus.  The
> >product is a capsule that dissolves almost completely if the broth is
> >warmed, but I don't think it would be "suitable" for membrane
> >filtration.  I could try filtering a portion of the broth or multiple
> >tube method if I needed to, but I'd like to avoid that if I could.  Any
> >other ideas?  Or do you think it would be safe to just monitor at 1:20
> >or 1:100?  Every other organism recovered fine at these dilutions.  Any
> >help would be appreciated.
> >
> >Curt Merideth
> >Microbiologist
> >
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>Veltek Associates, Inc - http://www.sterile.com
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>=================================
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>EMSL Analytical, Inc. - http://www.emsl.com
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>
>MIDI, Inc. - http://www.midi-inc.com
>
>Millipore - http://www.millipore.com
>
>Pall - http://www.pall.com
>
>NovaTek International - http://www.ntint.com
>
>Raven Biological Labs - http://www.ravenlabs.com
>
>Veltek Associates, Inc - http://www.sterile.com
>
>=================================
>The nature of this service is to provide a medium for communication.  The 
>specific statements and endorsements of individuals participating in the 
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The PMFList (http://microbiol.org/PMFList_info.htm) is operated from
The Microbiology Network (http://microbiol.org) and supported by
our sponsors (http://microbiol.org/sponsor.htm) as a service to
the scientific community.

Please take a second to visit our sponsors' web sites and say thank you for their support of this service.

Accugenix - http://www.accugenix.com

American Type Culture Collection - http://www.atcc.org

ATS Laboratories - http://www.ats-labs.com

Biolog - http://www.biolog.com

Biomerieux - http://industry.biomerieux-usa.com

Dupont Qualicon - http://www.qualicon.com

EMD Chemicals - http://www.emdchemicals.com

EMSL Analytical, Inc. - http://www.emsl.com

Genomic Profiling Systems, Inc. - http://www.genprosys.com

MIDI, Inc. - http://www.midi-inc.com

Millipore - http://www.millipore.com

Pall - http://www.pall.com

NovaTek International - http://www.ntint.com

Raven Biological Labs - http://www.ravenlabs.com

Veltek Associates, Inc - http://www.sterile.com

=================================
The nature of this service is to provide a medium for communication.  The specific statements and endorsements of individuals participating in the discussions are not necessarily those of The Microbiology Network, Inc., the PMF, or the sponsors of the list.

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