PMFLIST Archives

May 2006


Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
stacey chan <[log in to unmask]>
Reply To:
The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>
Wed, 24 May 2006 15:24:46 +0800
text/plain (72 lines)
Thanx a million Brendan and Dan!!

 I was not able to open the pdf file.Can you please resend to me?By the
way,what QA book are you refering to?As far as I am concern Duran is a
glassware brand:p Can't find anything related to QA stuff on the net.

For question 1: If you say you add 10ml of product to 190ml of
diluent,wouldn't the ratio for sample prep be 1:20?Then it is't applying to
the std method which uses 1:10.May I know is the 1:50 ratio determined by
trial & error?How do you come to that ratio then?I don't find it in the

*   Dan:*
There was a typo error in the last sentence of the question.What I meant was
'So,when I don't find it in the pharmacopoeia I'm transfering 0.1ml of the
contents into 100ml broth,is the concentration1g/ml?But anyway, since you
explain that '  The principle of methods is to revigorate enterobacteria so
the sample is diluted directly in broth medium ...' I should be transfering
10ml (since its actually dilution even its added to enrich. broth).
10g sample + 90ml broth D-------0.1g product in 1ml
                so;1g or ml --------0.1 x *10ml *+ 100ml broth E

For question 6:
What is Harmonised method? I don't find it in the pharmacopoeia.

Hope to hear from all soon.Thank you!


The PMFList ( is operated from
The Microbiology Network ( and supported by
our sponsors ( as a service to
the scientific community.

Please take a second to visit our sponsors' web sites and say thank you for their support of this service.

Accugenix -

American Type Culture Collection -

ATS Laboratories -

Biolog -

Biomerieux -

Dupont Qualicon -

EMD Chemicals -

EMSL Analytical, Inc. -

Genomic Profiling Systems, Inc. -

MIDI, Inc. -

Millipore -

Pall -

NovaTek International -

Raven Biological Labs -

Veltek Associates, Inc -

The nature of this service is to provide a medium for communication.  The specific statements and endorsements of individuals participating in the discussions are not necessarily those of The Microbiology Network, Inc., the PMF, or the sponsors of the list.