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October 2009

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Subject:
From:
Don English <[log in to unmask]>
Reply To:
The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>
Date:
Wed, 14 Oct 2009 10:36:06 -0400
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Dear Ed,

      I find it a little surprising that you are not able to neutralize the
residues of 6% Hydrogen peroxide from disinfectant efficacy testing.  It
was my understanding that residues of Hydrogen peroxide breaks down into
water and Oxygen.    At another position that I had held in the past, I had
used Catalase in the microbial count diluents and enrichments broths to
neutralize the antimicrobial activity from tooth whitening finished
products containing hydrogen peroxide, but the concentration of Hydrogen
peroxide was higher (e.g. (10 to 22%).  However, I do not recall the use
concentration of Catalase at this time.  For your information, Catalase is
available from Sigma.

Don




                                                                           
             "Balkovic, Ed"                                                
             <Ed.Balkovic@GENZ                                             
             YME.COM>                                                   To 
             Sent by: The              [log in to unmask]         
             Pharmaceutical                                             cc 
             Microbiology                                                  
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             <[log in to unmask]         [PMFLIST] How to neutralize 6% H2O2 
             CROBIOL.ORG>              for disinfectant efficacy testing?  
                                                                           
                                                                           
             10/14/2009 07:09                                              
             AM                                                            
                                                                           
                                                                           
             Please respond to                                             
                    The                                                    
              Pharmaceutical                                               
               Microbiology                                                
             Forum Email List                                              
             <[log in to unmask]                                             
               CROBIOL.ORG>                                                
                                                                           
                                                                           




Fellow Microbiologists:

Does anyone have a procedure to neutralize 6% hydrogen peroxide (H2O2)
for disinfectant efficacy testing?

We are having a problem recovering one of our test bacteria in the
stasis testing in samples containing 6% H2O2.
We are having to add a significant dilution factor for this bug and it
is affecting our ability to analyze the level of killing.

Thanks in advance,
ED

Ed Balkovic, Ph.D.
Quality Control Technical Services
Genzyme Corporation
[log in to unmask]

The opinions expressed above are my own and may not reflect those of my
employer.



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------------------
The PMFList (http://microbiol.org/PMFList_info.htm) is operated from
The Microbiology Network (http://microbiol.org) and supported by
our sponsors (http://microbiol.org/sponsor.htm) as a service to
the scientific community.

Please take a second to visit our sponsors' web sites and say thank you for their support of this service.

Accugenix - http://www.accugenix.com

American Type Culture Collection - http://www.atcc.org

Applied Biosystems, MicroSeq Microbial ID System - http://www.microseq.com/

BD Diagnostic Systems - http://www.bd.com/ds/

Biolog - http://www.biolog.com

Biomerieux - http://industry.biomerieux-usa.com

EMD Chemicals - http://www.emdchemicals.com

MicroBioLogics, Inc. - http://www.microbiologics.com

Millipore - http://www.millipore.com

MODA Technology Partners - http://www.modatp.com

Pall - http://www.pall.com

NovaTek International - http://www.ntint.com

Rapid Micro Biosystems - http://www.rapidmicrobio.com  (formerly GPS)

Raven Biological Labs - http://www.ravenlabs.com

Veltek Associates, Inc - http://www.sterile.com

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The nature of this service is to provide a medium for communication.  The specific statements and endorsements of individuals participating in the discussions are not necessarily those of The Microbiology Network, Inc., the PMF, or the sponsors of the list.

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