PMFLIST Archives

May 2011


Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
nikunj chhag <[log in to unmask]>
Reply To:
The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>
Tue, 3 May 2011 12:58:55 +0530
text/plain (68 lines)
Dear Prakash,

At first, as per the regulatory standards, any National level regulated
culture collection centres are valid and acceptable to receive the standard
cultures for industrial use (like GPT of media and validation studies) e.g.
MTCC, NCL etc. in INDIA.

Secondly better to store the cultures using Cryopreservation technique
(storing the prepared stock cryo cultures at about -80 C for infinitve time
as per ATCC literatures) then the conventional slant technique. Even you can
get the working cultures of as low as 3rd passage as per the procedure set
forth, for the said purpose. (NOTE: It is vital to use the lowest possible
passage in testing to get the best microbial response). However,
for your procedure it can be demonstrated through a validation study for the
storing of slant cultures.

You need to consider few things for this validation, like drying of agar
slants, viability of the stored cultures, and of course the storage time
period (in general 1 to 3 months is demonstratable; you have to give
transfer of cultures to fresh slants every 1 to 3 months). beyond six months
of storage of slant medium it was seen that the slants got dried.

Hope this will be useful to you. Regards.

Nikunj Chhag,
Famy Care Ltd.
Ahmedabad, Gujarat, India.

The PMFList ( is operated from
The Microbiology Network ( and supported by
our sponsors ( as a service to
the scientific community.

Please take a second to visit our sponsors' web sites and say thank you for their support of this service.

Accugenix -

American Type Culture Collection -

BD Diagnostic Systems -

Biolog -

Biomerieux -

Biotest -

MicroBioLogics, Inc. -

Millipore -

MODA Technology Partners: now a LONZA company

Pall -

NovaTek International -

Rapid Micro Biosystems -  (formerly GPS)

Veltek Associates, Inc -

The nature of this service is to provide a medium for communication.  The specific statements and endorsements of individuals participating in the discussions are not necessarily those of The Microbiology Network, Inc., the PMF, or the sponsors of the list.