Dear Aparna,
For the identification of anaerobic bacteria, a 15% Hydrogen peroxide
solution is necessary to perform a Catalase Test.. The reference for this
15% Hydrogen peroxide test is as follows:
Barlelt, M. 2000 Diagnostic bacteriology, a study guide. F.A, Davis
Co., Philadelphia, Pa.
Don
From: aparna jagadale <[log in to unmask]>
To: [log in to unmask],
Date: 09/27/2012 04:23 PM
Subject: [PMFLIST] Catalase test
Sent by: The Pharmaceutical Microbiology Forum Email List
<[log in to unmask]>
Dear forum mebers,
In USP35 NF 30, general chapter<2022>, test for absence of Clostridium
species says catalase test to be done using "dilute hydrogen peroxide
solution".Can anyone please guide "dilute" means what is the percentage
concentration of hydrogen peroxide to use?
Also, what percentage of hydrogen peroxide is generally recommended for
catalse test of anaerobes.
Regards,
Aparna Jagadale
------------------
The PMFList (http://microbiol.org/PMFList_info.htm) is operated from
The Microbiology Network (http://microbiol.org) and supported by
our sponsors (http://microbiol.org/sponsor.htm) as a service to
the scientific community.
Please take a second to visit our sponsors' web sites and say thank you for
their support of this service.
Accugenix - http://www.accugenix.com
American Type Culture Collection - http://www.atcc.org
BD Diagnostic Systems - http://www.bd.com/ds/
Biolog - http://www.biolog.com
Biomerieux - http://industry.biomerieux-usa.com
EMD Millipore - http://bit.ly/Udi4CP
MicroBioLogics, Inc. - http://www.microbiologics.com
Pall - http://www.pall.com
NovaTek International - http://www.ntint.com
Rapid Micro Biosystems - http://www.rapidmicrobio.com (formerly GPS)
Steris - http://www.sterislifesciences.com/
Veltek Associates, Inc - http://www.sterile.com
=================================
The nature of this service is to provide a medium for communication. The
specific statements and endorsements of individuals participating in the
discussions are not necessarily those of The Microbiology Network, Inc.,
the PMF, or the sponsors of the list.
------------------
The PMFList (http://microbiol.org/PMFList_info.htm) is operated from
The Microbiology Network (http://microbiol.org) and supported by
our sponsors (http://microbiol.org/sponsor.htm) as a service to
the scientific community.
Please take a second to visit our sponsors' web sites and say thank you for their support of this service.
Accugenix - http://www.accugenix.com
American Type Culture Collection - http://www.atcc.org
BD Diagnostic Systems - http://www.bd.com/ds/
Biolog - http://www.biolog.com
Biomerieux - http://industry.biomerieux-usa.com
EMD Millipore - http://bit.ly/Udi4CP
MicroBioLogics, Inc. - http://www.microbiologics.com
Pall - http://www.pall.com
NovaTek International - http://www.ntint.com
Rapid Micro Biosystems - http://www.rapidmicrobio.com (formerly GPS)
Steris - http://www.sterislifesciences.com/
Veltek Associates, Inc - http://www.sterile.com
=================================
The nature of this service is to provide a medium for communication. The specific statements and endorsements of individuals participating in the discussions are not necessarily those of The Microbiology Network, Inc., the PMF, or the sponsors of the list.
|
