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November 2013

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From:
"Machuca-Perez, Keysa" <[log in to unmask]>
Reply To:
The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>
Date:
Mon, 25 Nov 2013 19:16:52 +0000
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From previous experiences, as part of the qualification of the equipment's to be used in a Micro Lab, we qualified a water bath at a temperature range of 45-48C, targeted the temperature at 46C.  The USP Pharmacopoeia was referenced, and the following information was found in Chapter 1117, Microbiology Best Laboratory Practices, "The molten agar medium should be held in a monitored water bath at a temperature of 45C to 50C for no more than 8 hours".   This temperature (45-48C) helps preventing the media to solidify (due to low temperatures) or denaturalization (due to high temperatures) and ensure that your media temperature will not affect your organism recovery.

Keysa

-----Original Message-----
From: The Pharmaceutical Microbiology Forum Email List [mailto:[log in to unmask]] On Behalf Of Peter Lisecki
Sent: Friday, November 22, 2013 3:42 PM
To: [log in to unmask]
Subject: Re: [PMFLIST] Water Bath Alternatives for Agar

Ali,     55-60 is definitely way too hot to store media.  Peter


On Sat, Nov 16, 2013 at 5:19 AM, ali raza <[log in to unmask]> wrote:

> Hi Ann;
>
> For your asked question, if you place agar medium in dry heat 
> sterilizer at 55 - 60 degree centigrade then you can remain agar 
> medium in liquid state. but before follow this you have to validate it 
> by performing growth promotion test at your defined time intervals.
>
> Ali Raza Kureishi
>
> A.M. Microbiology Laboratory,
> Ferozsons Laboratories Limited.,
> Aman Garrh,
> Nowshera, KPK.
> Pakistan.
>
>
>
>
>
>
> On Friday, November 15, 2013 7:35 AM, Anne Haven 
> <[log in to unmask]>
> wrote:
>
> Hello PMF forum,
>
> Looking for an alternative to a water bath or steam bath to keep agar 
> molten.  We unsuccessfully tried to qualify a bead bath to keep melted 
> agar before use.  Any suggestions would be appreciated.
>
> Regards,
> Anne
>
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> =================================
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> The specific statements and endorsements of individuals participating 
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> Network, Inc., the PMF, or the sponsors of the list.
>
> ------------------
> The PMFList (http://microbiologynetwork.com/pmflist.asp) is operated 
> from The Microbiology Network (http://microbiologynetwork.com) and 
> supported by our sponsors as a service to the scientific community.
>
> Please take a second to visit our sponsors' web sites and say thank 
> you for their support of this service.
>
>
> Life Technologies - http://www.lifetechnologies.com/PharmaAnalytics
>
> Accugenix - http://www.accugenix.com
>
> American Type Culture Collection - http://www.atcc.org
>
> ATS Labs - http://www.ats-labs.com
>
> BD Diagnostic Systems - http://www.bd.com/ds/
>
> Biolog - http://www.biolog.com
>
> EMD Millipore -  http://www.emdmillipore.com/biomonitoring
>
> MicroBioLogics, Inc. - http://www.microbiologics.com
>
> Microtest Laboratories, Inc. - http://www.microtestlabs.com
>
> Pall - http://www.pall.com
>
> NovaTek International - http://www.ntint.com
>
> Rapid Micro Biosystems - http://www.rapidmicrobio.com  (formerly GPS)
>
> Steris - http://www.sterislifesciences.com/
>
> Veltek Associates, Inc - http://www.sterile.com
>
> =================================
> The nature of this service is to provide a medium for communication.  
> The specific statements and endorsements of individuals participating 
> in the discussions are not necessarily those of The Microbiology 
> Network, Inc., the PMF, or the sponsors of the list.
>

------------------
The PMFList (http://microbiologynetwork.com/pmflist.asp) is operated from The Microbiology Network (http://microbiologynetwork.com) and supported by our sponsors as a service to the scientific community.

Please take a second to visit our sponsors' web sites and say thank you for their support of this service.


Life Technologies - http://www.lifetechnologies.com/PharmaAnalytics

Accugenix - http://www.accugenix.com

American Type Culture Collection - http://www.atcc.org

ATS Labs - http://www.ats-labs.com

BD Diagnostic Systems - http://www.bd.com/ds/

Biolog - http://www.biolog.com

EMD Millipore -  http://www.emdmillipore.com/biomonitoring

MicroBioLogics, Inc. - http://www.microbiologics.com

Microtest Laboratories, Inc. - http://www.microtestlabs.com

Pall - http://www.pall.com

NovaTek International - http://www.ntint.com

Rapid Micro Biosystems - http://www.rapidmicrobio.com  (formerly GPS)

Steris - http://www.sterislifesciences.com/

Veltek Associates, Inc - http://www.sterile.com

=================================
The nature of this service is to provide a medium for communication.  The specific statements and endorsements of individuals participating in the discussions are not necessarily those of The Microbiology Network, Inc., the PMF, or the sponsors of the list.

------------------
The PMFList (http://microbiologynetwork.com/pmflist.asp) is operated from
The Microbiology Network (http://microbiologynetwork.com) and supported by
our sponsors as a service to the scientific community.

Please take a second to visit our sponsors' web sites and say thank you for their support of this service.


Life Technologies - http://www.lifetechnologies.com/PharmaAnalytics

Accugenix - http://www.accugenix.com

American Type Culture Collection - http://www.atcc.org

ATS Labs - http://www.ats-labs.com

BD Diagnostic Systems - http://www.bd.com/ds/

Biolog - http://www.biolog.com

EMD Millipore -  http://www.emdmillipore.com/biomonitoring

MicroBioLogics, Inc. - http://www.microbiologics.com

Microtest Laboratories, Inc. - http://www.microtestlabs.com

Pall - http://www.pall.com

NovaTek International - http://www.ntint.com

Rapid Micro Biosystems - http://www.rapidmicrobio.com  (formerly GPS)

Steris - http://www.sterislifesciences.com/

Veltek Associates, Inc - http://www.sterile.com

=================================
The nature of this service is to provide a medium for communication.  The specific statements and endorsements of individuals participating in the discussions are not necessarily those of The Microbiology Network, Inc., the PMF, or the sponsors of the list.

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