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The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>
Wed, 19 Apr 2017 02:43:15 -0400
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Dear Forum,

Does anyone have any experience of neutralizing ascorbic acid?

I am trying to validate the bioburden test (pre-sterile filtration bioburden) for a product that contains 50mg/ml of ascorbic acid, but it is inhibiting the growth of Pseudomonas aeruginosa. I have tried neutralization with MgCl2 but the results have been somewhat inconclusive. The product cannot be diluted enough without compromising the sensitivity of the test method, and I can't use membrane filtration. Do you have any suggestions what would be a suitable method and/or a neutralizer?

Thank you in advance

Best regards,

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