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April 2017

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Subject:
From:
Timothy Cser <[log in to unmask]>
Reply To:
The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>
Date:
Fri, 21 Apr 2017 16:28:23 +0000
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Have you tried sodium bicarbonate?

Tim Cser

MilliporeSigma

MilliporeSigma is a division of Merck KGaA, Darmstadt, Germany


-----Original Message-----
From: The Pharmaceutical Microbiology Forum Email List [mailto:[log in to unmask]] On Behalf Of Joan
Sent: Tuesday, April 18, 2017 11:43 PM
To: [log in to unmask]
Subject: [PMFLIST] Neutralizer for ascorbic acid (bioburden test)

Dear Forum,

Does anyone have any experience of neutralizing ascorbic acid?

I am trying to validate the bioburden test (pre-sterile filtration bioburden) for a product that contains 50mg/ml of ascorbic acid, but it is inhibiting the growth of Pseudomonas aeruginosa. I have tried neutralization with MgCl2 but the results have been somewhat inconclusive. The product cannot be diluted enough without compromising the sensitivity of the test method, and I can't use membrane filtration. Do you have any suggestions what would be a suitable method and/or a neutralizer?

Thank you in advance

Best regards,
Joan

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The PMFList (http://microbiologynetwork.com/pmflist.asp) is operated from
The Microbiology Network (http://microbiologynetwork.com) and supported by
our sponsors as a service to the scientific community.

Please take a second to visit our sponsors' web sites and say thank you for their support of this service.


Veltek Associates, Inc - http://www.sterile.com

Microbiologics, Inc. - http://www.microbiologics.com

Nelson Labs - http://www.nelsonlabs.com

BD Diagnostic Systems - http://www.bd.com/ds/

Boston Analytical http://www.bostonanalytical.com/

MilliporeSigma  http://www.emdmillipore.com/

Charles River Microbial Solutions:  Endosafe, Accugenix & Celsis   - www.criver.com/micro

Avista Pharma Solutions  http://www.avistapharma.com

Associates of Cape Cod, Inc. - http://www.acciusa.com/


=================================
The nature of this service is to provide a medium for communication.  The specific statements and endorsements of individuals participating in the discussions are not necessarily those of The Microbiology Network, Inc., the PMF, or the sponsors of the list.

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