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May 2018

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Subject:
From:
TINDALL Brendan <[log in to unmask]>
Reply To:
The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>
Date:
Tue, 29 May 2018 02:13:01 +0000
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Ashok,

Should be approximately 1,000,000 CFU per ml. The BIOBALL instructions for use recommends .1ml addition to 20mL of product for 500,000 cfu per ml.

(100,920,000 /1.1 )*.2  = 18,349,090CFU (rehydration)
18,349,090CFU /20.2ml = 908,380CFU per ml

Thanks,

Brendan


-----Original Message-----
From: The Pharmaceutical Microbiology Forum Email List [mailto:[log in to unmask]] On Behalf Of Ashok Udigiri
Sent: Monday, 28 May 2018 4:13 PM
To: [log in to unmask]
Subject: [PMFLIST] PET Calculation

Dear forum members,

For PET testing , we are using bioballs. On CoA for A. bresiliensis, mean CFU is 100920000. We reconstituted in 1.1 ml of reconstitution fluid and added 0.2 ml of culture to 20 ml of product. What is final concentration of culture in product per ml? Please explain.

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The PMFList (http://microbiologynetwork.com/pmflist.asp) is operated from
The Microbiology Network (http://microbiologynetwork.com) and supported by
our sponsors as a service to the scientific community.

Please take a second to visit our sponsors' web sites and say thank you for their support of this service.
If your company would be interested in sponsoring this community, please contact [log in to unmask]

Sartorius https://promotions.sartorius.com/real-heroes

Steris - http://www.sterislifesciences.com/

Avista Pharma Solutions  http://www.avistapharma.com

MilliporeSigma  http://www.emdmillipore.com/

Charles River Laboratories - http://www.criver.com/

Veltek Associates, Inc - http://www.sterile.com

Microbiologics, Inc. - http://www.microbiologics.com

Nelson Labs - http://www.nelsonlabs.com

BD Diagnostic Systems - http://www.bd.com/ds/

Boston Analytical http://www.bostonanalytical.com/

Associates of Cape Cod, Inc. - http://www.acciusa.com/


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The nature of this service is to provide a medium for communication.  The specific statements and endorsements of individuals participating in the discussions are not necessarily those of The Microbiology Network, Inc., the PMF, or the sponsors of the list.

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