Dear forum members,
during the annual autoclave qualification we used geobacillus strips for
the qualification of the decontamination cycle (131C). growth was observed
on the 1st day of incubation (the strips were placed inside the bag
containing contaminated dishes, inside the contaminated glass bottle and
inside the contaminated filtration device). we subcultured into fresh TSA
but there was no growth for none of them. the whole procedure was repeated
and exactly the same happened again.
any ideas what the original TSB turbidity might be? how shall i proceed to
further investigation of this incident?
thank you all in advance
Supervisor of the microbiological Department
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