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May 2018

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Subject:
From:
Evangelia Georgiadou <[log in to unmask]>
Reply To:
The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>
Date:
Wed, 9 May 2018 22:13:47 +0300
Content-Type:
text/plain
Parts/Attachments:
text/plain (168 lines)
yes the TSA were incubated at the same temperature as the spore strips.

the bottles were not sealed tight. this is what we actually do in our
routine work.

the specific cycle has already been validated the previous years using the
exact same procedure .

I honestly cannot make any assumptions on what may have happened!

On Wednesday, May 9, 2018, Knutsen, Chris <[log in to unmask]> wrote:

> This might seem evident, but did you incubate the streaked plates at the
> elevated temperature?
>
> If you had the strips in bottles, etc., are you sure that there the
> pressurized steam can reach the spore strips?
>
>
> Chris Knutsen, Ph.D.
> Associate Director, Microbiology
> ABO Microbiology Science and Technology
> Bristol-Myers Squibb Company
> 1 Squibb Drive
> New Brunswick, NJ 08902
> Tel : +1-732-227-5375
> Fax: +1-732-227-3071
> Cell: +1-609-751-8362
> e-mail: [log in to unmask]
>
> -----Original Message-----
> From: The Pharmaceutical Microbiology Forum Email List <
> [log in to unmask]> On Behalf Of Evangelia Georgiadou
> Sent: Wednesday, May 9, 2018 1:22 AM
> To: [log in to unmask]
> Subject: [PMFLIST] autoclave validation
>
> Dear forum members,
>
> during the annual autoclave qualification we used geobacillus strips for
> the qualification of the decontamination cycle (131C). growth was observed
> on the 1st day of incubation (the strips were placed inside the bag
> containing contaminated dishes, inside the contaminated glass bottle and
> inside the contaminated filtration device). we subcultured into fresh TSA
> but there was no growth for none of them. the whole procedure was repeated
> and exactly the same happened again.
>
> any ideas what the original TSB turbidity might be? how shall i proceed to
> further investigation of this incident?
>
> thank you all in advance
> Evangelia Georgiadou
> Molecular Biologist
> Supervisor of the microbiological Department
>
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> =================================
> The nature of this service is to provide a medium for communication.  The
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> ------------------
> The PMFList (http://microbiologynetwork.com/pmflist.asp) is operated from
> The Microbiology Network (http://microbiologynetwork.com) and supported by
> our sponsors as a service to the scientific community.
>
> Please take a second to visit our sponsors' web sites and say thank you
> for their support of this service.
> If your company would be interested in sponsoring this community, please
> contact [log in to unmask]
>
> Sartorius https://promotions.sartorius.com/real-heroes
>
> Steris - http://www.sterislifesciences.com/
>
> Avista Pharma Solutions  http://www.avistapharma.com
>
> MilliporeSigma  http://www.emdmillipore.com/
>
> Charles River Laboratories - http://www.criver.com/
>
> Veltek Associates, Inc - http://www.sterile.com
>
> Microbiologics, Inc. - http://www.microbiologics.com
>
> Nelson Labs - http://www.nelsonlabs.com
>
> BD Diagnostic Systems - http://www.bd.com/ds/
>
> Boston Analytical http://www.bostonanalytical.com/
>
> Associates of Cape Cod, Inc. - http://www.acciusa.com/
>
>
> =================================
> The nature of this service is to provide a medium for communication.  The
> specific statements and endorsements of individuals participating in the
> discussions are not necessarily those of The Microbiology Network, Inc.,
> the PMF, or the sponsors of the list.
>

------------------
The PMFList (http://microbiologynetwork.com/pmflist.asp) is operated from
The Microbiology Network (http://microbiologynetwork.com) and supported by
our sponsors as a service to the scientific community.

Please take a second to visit our sponsors' web sites and say thank you for their support of this service.
If your company would be interested in sponsoring this community, please contact [log in to unmask]

Sartorius https://promotions.sartorius.com/real-heroes

Steris - http://www.sterislifesciences.com/

Avista Pharma Solutions  http://www.avistapharma.com

MilliporeSigma  http://www.emdmillipore.com/

Charles River Laboratories - http://www.criver.com/

Veltek Associates, Inc - http://www.sterile.com

Microbiologics, Inc. - http://www.microbiologics.com

Nelson Labs - http://www.nelsonlabs.com

BD Diagnostic Systems - http://www.bd.com/ds/

Boston Analytical http://www.bostonanalytical.com/

Associates of Cape Cod, Inc. - http://www.acciusa.com/


=================================
The nature of this service is to provide a medium for communication.  The specific statements and endorsements of individuals participating in the discussions are not necessarily those of The Microbiology Network, Inc., the PMF, or the sponsors of the list.

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