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May 2018

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Subject:
From:
Elizabeta Popovska <[log in to unmask]>
Reply To:
The Pharmaceutical Microbiology Forum Email List <[log in to unmask]>
Date:
Wed, 30 May 2018 12:48:20 +0200
Content-Type:
text/plain
Parts/Attachments:
text/plain (174 lines)
Hello Ashok,

Here is the concrete calculation:

100.920.000 CFU reconstituted in 1,1ml reconstitution fluid

18.349.090 CFU  in 0,2ml

18.349.090 CFU ( 0,2 ml are added) in 20ml product = 917.454 CFU/ml

You have approx. 900.000 cfu/ml

Regards

On Tue, May 29, 2018 at 7:33 AM, BISHT Kirti <[log in to unmask]>
wrote:

> For Bioball 10E8, the 100 microL aliquot provides a target concentration
> of ~500,000 cfu per mL when inoculated in 20 mL of sample product.
>
>
>
>
> Thanks & Regards
> Kirti BISHT
> bioMérieux India Pvt Ltd. | Industrial Microbiology |
> 43A, Okhla Industrial Estate, Okhla Phase III, New Delhi-110020
> Tel: +91 11 42098890 | Mobile: +91 8860077364
> www.biomerieux.com
>
>
>
> -----Original Message-----
> From: The Pharmaceutical Microbiology Forum Email List [mailto:
> [log in to unmask]] On Behalf Of Ashok Udigiri
> Sent: Monday, May 28, 2018 11:43 AM
> To: [log in to unmask]
> Subject: [PMFLIST] PET Calculation
>
> Dear forum members,
>
> For PET testing , we are using bioballs. On CoA for A. bresiliensis, mean
> CFU is 100920000. We reconstituted in 1.1 ml of reconstitution fluid and
> added 0.2 ml of culture to 20 ml of product. What is final concentration of
> culture in product per ml? Please explain.
>
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> Steris - http://www.sterislifesciences.com/
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> Avista Pharma Solutions  http://www.avistapharma.com
>
> MilliporeSigma  http://www.emdmillipore.com/
>
> Charles River Laboratories - http://www.criver.com/
>
> Veltek Associates, Inc - http://www.sterile.com
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> Microbiologics, Inc. - http://www.microbiologics.com
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The PMFList (http://microbiologynetwork.com/pmflist.asp) is operated from
The Microbiology Network (http://microbiologynetwork.com) and supported by
our sponsors as a service to the scientific community.

Please take a second to visit our sponsors' web sites and say thank you for their support of this service.
If your company would be interested in sponsoring this community, please contact [log in to unmask]

Sartorius https://promotions.sartorius.com/real-heroes

Steris - http://www.sterislifesciences.com/

Avista Pharma Solutions  http://www.avistapharma.com

MilliporeSigma  http://www.emdmillipore.com/

Charles River Laboratories - http://www.criver.com/

Veltek Associates, Inc - http://www.sterile.com

Microbiologics, Inc. - http://www.microbiologics.com

Nelson Labs - http://www.nelsonlabs.com

BD Diagnostic Systems - http://www.bd.com/ds/

Boston Analytical http://www.bostonanalytical.com/

Associates of Cape Cod, Inc. - http://www.acciusa.com/


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The nature of this service is to provide a medium for communication.  The specific statements and endorsements of individuals participating in the discussions are not necessarily those of The Microbiology Network, Inc., the PMF, or the sponsors of the list.

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