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October 1998


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"MICHAEL CARROLL (Sr. QA Mgr., Osteotech, Inc." <[log in to unmask]>
Reply To:
The Pharmaceutical Microbiology Mail List <[log in to unmask]>
Wed, 21 Oct 1998 07:41:24 -0500
text/plain (96 lines)
     I know of no PROGRAM (= software?) that does this; however, if it
     exists, Kaye Instruments is probably a source, if not its developer.
     Their equipment has done these calculations forever....Them, or
     Steris, who seems to be cornering the market on sterilization systems
     of all types (steam, VHP, etc.). Otherwise, in old-fashioned PAPER
     form, I refer you to the PDA Technical Monograph No. 1 on Validation
     of Steam Sterilization Cycles-- has all the formulae and tables you'd
     need to develop your OWN software via Lotus or Excel or some such...
        Hope this is SOME help-
        M.Carroll ([log in to unmask]).

______________________________ Reply Separator _________________________________
Subject: [PMFLIST] CAL program for calculation of Fo values?
Author:  <[log in to unmask]> at INTERNET-MAIL
Date:    10/20/98 5:07 PM

Dear All,

As an academic pharmacist involved in teaching pharmaceutical microbiology,
I find the
new discussion group both interesting and useful.

Could I ask the group if any of the members knows of a computer package or
web site
where there is a Computer Aided Learning program which deals with Fo values.?
I would specifically like a program which would show, graphically or
diagramatically, how to
calculate D values, Z values and ultimately Fo values.  We have a program
which takes
data from an autoclave and prints out Fo at one minute intervals but this
does not help with
showing our students the processes of calculation of D  and Z values for
organisms.  Ideally we would like a program that starts with diagrams of
plates which are
symbolic representations of the surface count method cfus from organisms
that have been
heated at given temperatures for given times.  From these the students
could  count cfus, then
calculate the D values and subsequently the Z values.  An even more useful
program would be
one that gave data that allowed the calculation for different species. A
comparison of the calculations for  a non-sporulating vegetative organism
compared with the spores of, say, B. stearothermophilus would also be very

We would also be interested in a similar type of program which allows the
calculation of the log reduction values obtained from "sterile" filtration
using membrane filters.

At present we do these calculations with one set of real plates in the lab.
but do not have time for repeats with different organisms or if anything
goes wrong.

If you know of any such programs please e-mail me at the address below.

Many thanks in advance.

Howard Pryce-Jones.
Dr. R. Howard Pryce-Jones       Tel:  (+44) (0)141 552 4400 Ext. 2506
                                        Tel:  (+44) (0)141 548 2506
Departmental                    Fax:  (+44) (0)141 552 6443
Department of Pharmaceutical Sciences,
Strathclyde Institute for Biomedical Sciences,
University of Strathclyde,
27 Taylor Street,
Glasgow,                                |E-mail:
G4  0NR,                                [log in to unmask]    or
Scotland, UK.                           [log in to unmask]

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