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November 1998


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rfriedel <[log in to unmask]>
Reply To:
Robert R. Friedel
Mon, 30 Nov 1998 08:15:32 -0500
text/plain (64 lines)
The media they are probably referring to is Presence/Absence Broth or a
similar  coliform detection media containing "MUG" reagent.

MUG or "Methylumbelliferyl-beta-D-glucuronide" is used to detect E. coli.
When this chemical is cleaved by glucuronidase (an enzyme specific to E.
coli),  a fluorogenic product is produced.  When examined under long-wave UV
light, the medium with fluoresce.

This chemical adjunct is recognized by USDA, EPA, and AOAC in testing for
the presence of E. coli.

Available thru:  The Hach Company, Loveland, Colorado (800) 227-4224

Robert R. Friedel
(formerly Research Microbiologist, Whitehall-Robins Healthcare)
Quality Assurance Manager
Laboratory Research & Analysis Groups
Perritt Laboratories, Inc.
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-----Original Message-----
From: Stanley Alekman <[log in to unmask]>
To: [log in to unmask] <[log in to unmask]>
Date: Thursday, November 26, 1998 4:07 PM
Subject: [PMFLIST] Q: E Coli testing

>I visited a water testing lab in Japan that does some micro testing. The
>communication was not good because I have no Japanese capability and the
>person I spoke to had limited english.
>This lab does coliform testing using what they call an enzyme specific
>and 366 nm illumination for visualization of E coli. Test time is 24 hours.
>They perform this test first, and in addition to the standard tests for E
>which take a week.
>Can someone tell me more about this test? The media? etc.
>Thank you.
>Stan Alekman
><[log in to unmask]>
>The PMFList ( is operated from
>The Microbiology Network ( and supported by
>our sponsors ( as a service to
>the scientific community.

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