Your experimental results are in agreement with the idea that I offered, which
is that it won't make much difference what temperature is used, assuming we
are talking about standard mesophilic range conditions. I didn't specify what
challenge bugs to use: pedigree or wild type. I should have. My approach
would again be the same as yours: use environmental/process isolates over
I do think that extended incubation, no matter the temperature, will cull out
more cfus up to a reasonable point. Of course, this causes problems when
relating to USP <71>.
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