Sorry, only just got round now to reading this. If you are referring to solid media such as Brewer's Agar, TSA, Colombia agar, etc., you should definitely incubate for sterility testing in anaerobic conditions. If not, then you have no way of knowing if the media is contaminated with obligatory anaerobes, as they will fail to grow, thus rendering your sterility check worthless. In general, the conditions for the sterility QC test should be similar to routine test conditions. For thioglycollate broth - the lower regions of the broth are meant to be inherently anaerobic without the need for an anaerobic jar. During routine use they are incubated in a normal aerobic incubator, and your QC sterility test should use similar conditions. Baruch Pinnick, Omrix Pharmaceuticals, ISRAEL ========= On Fri, Apr 15, 2011 at 7:16 PM, Luis Raices <[log in to unmask]> wrote: > In your experience: do you sterility test media intended for anaerobic organisms > under anaerobic conditions? > > Thanks, > > Luis ------------------ The PMFList (http://microbiol.org/PMFList_info.htm) is operated from The Microbiology Network (http://microbiol.org) and supported by our sponsors (http://microbiol.org/sponsor.htm) as a service to the scientific community. Please take a second to visit our sponsors' web sites and say thank you for their support of this service. Accugenix - http://www.accugenix.com American Type Culture Collection - http://www.atcc.org BD Diagnostic Systems - http://www.bd.com/ds/ Biolog - http://www.biolog.com Biomerieux - http://industry.biomerieux-usa.com Biotest - http://www.BiotestUSA.com/micro MicroBioLogics, Inc. - http://www.microbiologics.com MODA Technology Partners: now a LONZA company http://lonza.com/moda Pall - http://www.pall.com NovaTek International - http://www.ntint.com Rapid Micro Biosystems - http://www.rapidmicrobio.com (formerly GPS) Veltek Associates, Inc - http://www.sterile.com ================================= The nature of this service is to provide a medium for communication. The specific statements and endorsements of individuals participating in the discussions are not necessarily those of The Microbiology Network, Inc., the PMF, or the sponsors of the list.