Aparna, Had you 'googled' on this test, you would have come across this link, that tells you all about catalase tests for aerobes and anaerobes. http://www.microbelibrary.org/index.php/library/laboratory-test/3226-cat alase-test-protocol Lakshmi Sadasivan [215-781-6493] -----Original Message----- From: The Pharmaceutical Microbiology Forum Email List [mailto:[log in to unmask]] On Behalf Of aparna jagadale Sent: Thursday, September 27, 2012 5:06 AM To: [log in to unmask] Subject: [PMFLIST] Catalase test Dear forum mebers, In USP35 NF 30, general chapter<2022>, test for absence of Clostridium species says catalase test to be done using "dilute hydrogen peroxide solution".Can anyone please guide "dilute" means what is the percentage concentration of hydrogen peroxide to use? Also, what percentage of hydrogen peroxide is generally recommended for catalse test of anaerobes. Regards, Aparna Jagadale ------------------ The PMFList (http://microbiol.org/PMFList_info.htm) is operated from The Microbiology Network (http://microbiol.org) and supported by our sponsors (http://microbiol.org/sponsor.htm) as a service to the scientific community. Please take a second to visit our sponsors' web sites and say thank you for their support of this service. Accugenix - http://www.accugenix.com American Type Culture Collection - http://www.atcc.org BD Diagnostic Systems - http://www.bd.com/ds/ Biolog - http://www.biolog.com Biomerieux - http://industry.biomerieux-usa.com EMD Millipore - http://bit.ly/Udi4CP MicroBioLogics, Inc. - http://www.microbiologics.com Pall - http://www.pall.com NovaTek International - http://www.ntint.com Rapid Micro Biosystems - http://www.rapidmicrobio.com (formerly GPS) Steris - http://www.sterislifesciences.com/ Veltek Associates, Inc - http://www.sterile.com ================================= The nature of this service is to provide a medium for communication. The specific statements and endorsements of individuals participating in the discussions are not necessarily those of The Microbiology Network, Inc., the PMF, or the sponsors of the list. This message and any attachments is intended for the named addressee(s) only and may contain information that is privileged and/or confidential. If you receive this message in error, please delete it and immediately notify the sender. Any copying, dissemination or disclosure, either whole or partial, by a person who is not the named addressee is prohibited. We use virus scanning software but disclaim any liability for viruses or other devices which remain in this message or any attachments. ***************** Ce message, ainsi que toute piece jointe, est exclusivement adresse au(x) destinataire(s) nomme(s) et peut contenir des informations confidentielles. Si vous recevez ce message par erreur, merci de le detruire et d'en avertir immediatement l'emetteur. Toute copie, transmission ou divulgation, integrale ou partielle, par une personne qui n'est pas nommee comme destinataire est interdite. Nous utilisons un logiciel anti-virus mais nous denions toute responsabilite au cas ou des virus, ou tout autre procede, seraient contenus dans ce message ou toute piece jointe. ------------------ The PMFList (http://microbiol.org/PMFList_info.htm) is operated from The Microbiology Network (http://microbiol.org) and supported by our sponsors (http://microbiol.org/sponsor.htm) as a service to the scientific community. Please take a second to visit our sponsors' web sites and say thank you for their support of this service. Accugenix - http://www.accugenix.com American Type Culture Collection - http://www.atcc.org BD Diagnostic Systems - http://www.bd.com/ds/ Biolog - http://www.biolog.com Biomerieux - http://industry.biomerieux-usa.com EMD Millipore - http://bit.ly/Udi4CP MicroBioLogics, Inc. - http://www.microbiologics.com Pall - http://www.pall.com NovaTek International - http://www.ntint.com Rapid Micro Biosystems - http://www.rapidmicrobio.com (formerly GPS) Steris - http://www.sterislifesciences.com/ Veltek Associates, Inc - http://www.sterile.com ================================= The nature of this service is to provide a medium for communication. The specific statements and endorsements of individuals participating in the discussions are not necessarily those of The Microbiology Network, Inc., the PMF, or the sponsors of the list.