Hello Dear Forum Members, My query is when OOS observed in any MET Sample, we identify the particular strain and after that perform Propogation Test. Mine query is why we do Propogation Test what is this test means in Microbiology? And also what is the procedure. Another one is while performing GPT of Anaerobes we provide all anaerobic conditions like Anaerobic Jar and Gas Kit and to assure we add chemical indicator which indicates the whole process goes wright with colour change. So the indicator is covered with some wrapper so without take out from that wrapper how colour change done. Thanks Warm Regards ------------------ The PMFList (http://microbiologynetwork.com/pmflist.asp) is operated from The Microbiology Network (http://microbiologynetwork.com) and supported by our sponsors as a service to the scientific community. Please take a second to visit our sponsors' web sites and say thank you for their support of this service. If your company would be interested in sponsoring this community, please contact [log in to unmask] Science Advisory Board https://www.scienceboard.net/ Steris - http://www.sterislifesciences.com/ Charles River Laboratories - http://www.criver.com/ Veltek Associates, Inc - http://www.sterile.com Microbiologics, Inc. - http://www.microbiologics.com BD Industrial Media - http://www.bd.com/ds/ Boston Analytical http://www.bostonanalytical.com/ Associates of Cape Cod, Inc. - http://www.acciusa.com/ ================================= The nature of this service is to provide a medium for communication. The specific statements and endorsements of individuals participating in the discussions are not necessarily those of The Microbiology Network, Inc., the PMF, or the sponsors of the list.