Hi Jovan. What indicato are you using. In my experience, resazurin works better than methylene blue. Also, what box are you using? I was always partial to Mitsubishi. Finally, those seals are known for leaks so a more sensitive indicator may be helpful. Tim Cser 916-759-1401 [log in to unmask] > On Nov 26, 2019, at 7:41 AM, Jovan Rankov <[log in to unmask]> wrote: > > Dear forum, > > This a correction of my query, to avoid confusion. > Recently in our microlab we obrained growth of strictly aerobic species in our anaerobic jars. We then tested our anaerobic jars (with oxygen indicators) by incubating strictly aerobic species from our library of isolates, and we obtained full growth on all petri dishes. Indicators suggested that the atmosphere was anaerobic after a few hours. We even sent our strains to genetic ID and got the same results - species are strictly aerobic (Micrococcus luteus, Acinetobacter lwoffii). > > In GP test we use these jars for anaerobic strains and we do obtain growth of strictly anaerobic strains regularly (Clostridium spp.). > > We tested old and new jars, lids were tight, indicators were within shelf life, everything seemed allright. > > Does anyone else think this is weird? How would you proceed? > > Jovan > QC Microbiologist > > ________________________________ > > > Odricanje od odgovornosti: > Ovaj dokument namenjen je samo licima kojima je upu?en i za pozivanje na isti od strane bilo kog lica, neophodna je naknadna pismena potvrda njegovog sad?aja. Shodno tome, HEMOFARM A.D. Vrsac odrice svaku odgovornost i ne prihvata bilo kakvu obavezu (uklju?ujuci slu?aj nepa?nje) za posledice koje moze pretpeti bilo koje lice zbog ?injenja ili ne?injenja na bazi takve informacije pre nego ?to takva lica prime dodatnu pismenu potrvdu. 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