Tony, We use a 10 minute cycle (on high) for about a half liter of agar in 1 liter screw cap bottles. Can't remember what the wattage is, though. Of course, following in your footsteps, we validated the cycle by comparing growth promotion characteristics of microwaved agar vs. our standard autoclave remelt cycle, which was previously validated. Rule of thumb: we only remelt one time. Oh, one other thing: we can get two bags of popcorn done at the same time! Michael Dr. Michael J. Miller Director, Biological and Sterilization Sciences Bausch & Lomb [log in to unmask] ------------------ The PMFList (http://microbiol.org/PMFList_info.htm) is operated from The Microbiology Network (http://microbiol.org) and supported by our sponsors (http://microbiol.org/sponsor.htm) as a service to the scientific community.